Special topics in Structural Biology

The first structure of an Aldehyde dehydrogenase reveals novel interations between NAD and the Rossmann fold
 

Nature Structural Biology, 1997, 4(4), 317-326


A.  Introduction to aldehyde dehydrogenase
 

    Aldehyde DHase (ALDH) is an important emzyme in  alcohol metabolism , ALDH make acetaldehyde
convert into acetic acid, and these step (alcohol DHase, and aldehyde DHase) is " detoxication"steps in liver.


 

(I)ALDH catalytic mechanism

(II)And it exists as distinct enzymes with different tissue specificities, eyes , liver, kidney.....

Aldehyde DHase (ALDH) has three classes:
    class¢¹: cytosolic ALDH
    class¢º: mitochondrial ALDH
    class¢»: tumor associated

(In this paper"Nature structural biology" is Class ¢» in rat liver)
 
 

(III)Aldehyde DHase is homodimer, and have three domains:
       1. NAD-binding domain
       2. Catalytic domain
       3. Bridging domain

the star indicated the catalytic pocket site
 
 
 

(IV)Topology of ALDH

and its topology contains Rossmann fold(£]-£\-£])
 

[Next]  B. The details of ALDH structure