Lih-Yuan Lin PH. D.
(Cornell University, 1987)


Research interests

Molecular Biology, Biochemistry, Toxicology The major interest of our laboratory is to investigate the action of heavy metals (i.e., cadmium, zinc) on the cellular metabolisms, gene expressions and transcriptional regulations. We use metallothionein, a small molecular weight, cysteine rich, metal binding protein, as the target to monitor the responses occurred when heavy metals on the regulation of metallothionein gene expression is currently under investigation. Our laboratory is also interested in studying the structureal-functional relationship of a brain specific metallothionein isoform, MTIII. Unlike other metallothionein families, which are mainly involved in metal detoxification, MTIII reveals a specific neuron growth inhibitory effect. We cloned the MTIII cDNA from porcine brain and produced the protein in yeast. Using the synthesized MTIII, we are currently investigating its effect by primary neuronal culture. We are going to change certain amino acid residues by sitedirected mutagenesis and study the amino acids involved in the unique function of MTIII.

Representative Publications

  1. Chiu, C.-H., Lee, C.-Z., Lin, K.-S., Tam, M.F. and Lin, L.-Y. (1999) Amino acid residues involved in the functional integrity of Escherichia coli methionine aminopeptidase. J. Bacteriol. 15: 4686-4689
  2. Huang, D. D.-W., Liu, L.-F., Kuan, I-C., Lin, L.-Y., Tam, T.-C. S. and Tam, M.F. (1999) Co-expression glutathionine S-transferase with methionine aminopeptidase. A system of producing enriched N-terminal processed proteins in Escherchia coli. Biochem. J. 338:335-342
  3. Yu, C.-W., Chen, H.-C. and Lin, L.-Y. (1998) Transcription of metallothionein isoform promoters is differentially regulated in cadmium sensitive and resistant CHO cells. J. Cell. Biochem. 68:174-185
  4. Wang, S.-H., Shih, Y.-H. and Lin, L.-Y. (1998) Yeast consensus initiator sequence markedly enhances the synthesis of metallothionein III in Saccharomyces cerevisiae. Biotechnol. Lett. 20:9-13
  5. Yu. C.-W., Chen, J.-H. and Lin, L.-Y. (1997) Metal-induced metallothionein gene expresion can be inactivated by protein kinase C inhibitor. FEBS Lett. 420:69-73
  6. Wang, S.-H., Chang, C.-Y., Chen, C.-F., Tam, M.F., Shih, Y-H. and Lin, L-Y. (1997) Cloning of porcine neuron growth inhibitory factor (metallothionein III) cDNA and expression of the gene in Saccharomyces cerevisiae. Gene 203:189-197
  7. Chen, C.-F., Wang, S.-H. and Lin, L.-Y. (1996) Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain. Comp. Biochem. Physiol. 115B:27-32
  8. Lee, Y. J., Chen, Y, P., Wang S. H., Chou W. Y. and Lin, L. Y. (1996) Structure and expression of metallothionein gene in ducks. Gene
  9. Liu, S. T., Hu, Y. P., Wang, C. t. and Lin, L. Y. (1996) Sequence and comparison of duck mitochondrial DNA control regions. Comp. Biochem. Physiol.
  10. Chen, C. F., Wang, S. H. and Lin, L. Y. (1996) Genetic analysis of the glucose-6-phosphatase mutation of type 1a glycogen torage disease in a chinese family. Clin. Genet.
  11. Lee, W. J., Yang, C. H., Ho, E.S. C., Shih, A., L. Y. Lin and W. H. Lin. (1996) Prenatal diagnosis in a Chinese family with type 1a glycogen storage disease by PCR-based genetic analysis. Prenat. Diagn.
  12. Yu, C. W. and Lin, L. Y. (1995) Cloning and characterization of the 5'-flanking region of metallothionein-l gene from Chinese hamster ovary cells. Biochim. Biophys. Acta 1262:150-154
  13. Huang, L. C., Lin, L. Y., Chen, C. M., Chen, L. J. Huang, B. L. and Murashige T. (1995) phase reversal in sequoia sempervirens in relation to mthDNA. Physiol. Plant. 94:379-383
  14. Chiu, S. J., Hong, S. H., Lin, L. Y. and Pan, R. L. (1995) A cDNA clone encoding the A subunit of the vacuolar H+-pyrophosphatase cDNA from etiolated mung bean seedings. Plant. Physiol. 109:1125-1127
  15. Hong, S. H., Chiu, S. J. Lin, L. Y. and Pan, R. L. (1995) Vacuolar H+-pyrophosphatase cDNA from etiolated mung bean seedings. Plant. Physiol. 109:1125-1127
  16. Wang, S. H., Chen, J. H. and Lin, L. Y. (1994) Functional integrity of metallothionein genes in testicular cell lines. J. Cell. Biochem. 55:486-495