SAPS. Version of January 7, 1995. Date run: Tue Dec 10 21:42:58 1996 SWISS-PROT ANNOTATION: ID Q02454 DE Q02454, 81 bases. number of residues: 81; molecular weight: 9.1 kdal 1 MKTLLLTTVV VTIVCLDLEY TLKCNKLVPL FYKTCPAGKN LCYKMFMVAT PKVPVKRGCI 61 DVCPKSSLLV KYVCCNTDRC N -------------------------------------------------------------------------------- COMPOSITIONAL ANALYSIS (extremes relative to: swp23s.q) A : 2( 2.5%); C : 9(11.1%); D : 3( 3.7%); E : 1( 1.2%); F : 2( 2.5%) G : 2( 2.5%); H : 0( 0.0%); I : 2( 2.5%); K : 10(12.3%); L : 11(13.6%) M : 3( 3.7%); N : 4( 4.9%); P : 5( 6.2%); Q : 0( 0.0%); R : 2( 2.5%) S : 2( 2.5%); T : 8( 9.9%); V : 11(13.6%); W : 0( 0.0%); Y : 4( 4.9%) KR : 12 ( 14.8%); ED : 4 ( 4.9%); AGP : 9 ( 11.1%); KRED : 16 ( 19.8%); KR-ED : 8 ( 9.9%); FIKMNY : 25 ( 30.9%); LVIFM : 29 ( 35.8%); ST : 10 ( 12.3%). -------------------------------------------------------------------------------- CHARGE DISTRIBUTIONAL ANALYSIS 1 0+00000000 000000-0-0 00+00+0000 00+00000+0 000+000000 0+000++000 61 -000+00000 +000000-+0 0 A. CHARGE CLUSTERS. Positive charge clusters (cmin = 12/30 or 16/45 or 19/60): none Negative charge clusters: not evaluated (frequency of - < 5%, too low) Mixed charge clusters (cmin = 14/30 or 19/45 or 24/60): none B. HIGH SCORING (UN)CHARGED SEGMENTS. ______________________________________ High scoring positive charge segments: score= 2.00 frequency= 0.148 ( KR ) score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.802 ( LAGSVTIPNFQYHMCW ) score= -2.00 frequency= 0.049 ( ED ) Expected score/letter: -0.605 - now scoring for positive charge segments; Average information/letter: 0.727 Minimal length of displayed segments set to: 20 M_0.01= 11.01 (cv= 6.42, lambda= 0.68489, k= 0.23341, x= 4.59; 90% confidence interval for segment length: 15 +- 15) M_0.05= 8.63 (x= 2.21) # of segments (>=20 residues) exceeding M_0.05: none ______________________________________ High scoring negative charge segments: score= 2.00 frequency= 0.049 ( ED ) score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.802 ( LAGSVTIPNFQYHMCW ) score= -2.00 frequency= 0.148 ( KR ) Expected score/letter: -100.00 - now scoring for negative charge segments; Average information/letter: 2.722 Minimal length of displayed segments set to: 20 M_0.01= 5.91 (cv= 3.17, lambda= 1.38629, k= 0.44580, x= 2.74; 90% confidence interval for segment length: 4 +- 4) M_0.05= 4.73 (x= 1.56) # of segments (>=20 residues) exceeding M_0.05: none ___________________________________ High scoring mixed charge segments: score= 1.00 frequency= 0.198 ( KEDR ) score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.802 ( LAGSVTIPNFQYHMCW ) Expected score/letter: -0.605 - now scoring for mixed charge segments; Average information/letter: 1.223 Minimal length of displayed segments set to: 20 M_0.01= 5.86 (cv= 3.13, lambda= 1.40180, k= 0.45603, x= 2.72; 90% confidence interval for segment length: 10 +- 8) M_0.05= 4.69 (x= 1.56) # of segments (>=20 residues) exceeding M_0.05: none ________________________________ High scoring uncharged segments: score= 1.00 frequency= 0.802 ( LAGSVTIPNFQYHMCW ) score= 0.00 frequency= 0.000 ( BZX ) score= -8.00 frequency= 0.198 ( KEDR ) Expected score/letter: -0.778 - now scoring for uncharged segments; Average information/letter: 0.128 Minimal length of displayed segments set to: 20 M_0.01= 38.95 (cv= 26.39, lambda= 0.16653, k= 0.08143, x= 12.56; 90% confidence interval for segment length: 73 +- 63) M_0.05= 29.16 (x= 2.78) # of segments (>=20 residues) exceeding M_0.05: none C. CHARGE RUNS AND PATTERNS. pattern (+)| (-)| (*)| (0)| (+0)| (-0)| (*0)|(+00)|(-00)|(*00)| lmin0 5 | 3 | 5 | 34 | 9 | 6 | 10 | 11 | 7 | 12 | lmin1 6 | 4 | 7 | 41 | 11 | 8 | 12 | 13 | 9 | 15 | lmin2 7 | 5 | 8 | 45 | 12 | 9 | 14 | 15 | 10 | 16 | There are no charge runs or patterns exceeding the given minimal lengths. Run count statistics: + runs >= 3: 0 - runs >= 3: 0 * runs >= 4: 0 0 runs >= 22: 0 -------------------------------------------------------------------------------- DISTRIBUTION OF OTHER AMINO ACID TYPES 1. HIGH SCORING SEGMENTS. __________________________________ High scoring hydrophobic segments: 2.00 (LVIFM) 1.00 (AGYCW) 0.00 (BZX) -2.00 (PH) -4.00 (STNQ) -8.00 (KEDR) Expected score/letter: -1.469 - now scoring for hydrophobic segments ........40........80.......120.......160.......200 *******; Average information/letter: 0.372 Minimal length of displayed segments set to: 15 M_0.01= 27.80 (cv= 16.69, lambda= 0.26323, k= 0.18675, x= 11.10; 90% confidence interval for segment length: 28 +- 22) M_0.05= 21.60 (x= 4.91) # of segments (>=15 residues) exceeding M_0.05: none ____________________________________ High scoring transmembrane segments: 5.00 (LVIF) 2.00 (AGM) 0.00 (BZX) -1.00 (YCW) -2.00 (ST) -6.00 (P) -8.00 (H) -10.00 (NQ) -16.00 (KR) -17.00 (ED) Expected score/letter: -2.704 - now scoring for transmembrane segments ........40........80.......120.......160.......200 ********; Average information/letter: 0.323 Minimal length of displayed segments set to: 15 M_0.01= 60.71 (cv= 37.78, lambda= 0.11632, k= 0.14485, x= 22.94; 90% confidence interval for segment length: 32 +- 27) M_0.05= 46.70 (x= 8.92); M_0.30= 30.03 (x= -7.75) # of segments (>=15 residues) exceeding M_0.30: none 2. SPACINGS OF C. H2N-14-C-8-C-10-C-6-C-16-C-3-C-10 CC at 74 -4-C-1-COOH -------------------------------------------------------------------------------- REPETITIVE STRUCTURES. A. SEPARATED, TANDEM, AND PERIODIC REPEATS: amino acid alphabet. Repeat core block length: 4 B. SEPARATED AND TANDEM REPEATS: 11-letter reduced alphabet. (i= LVIF; += KR; -= ED; s= AG; o= ST; n= NQ; a= YW; p= P; h= H; m= M; c= C) Repeat core block length: 8 -------------------------------------------------------------------------------- MULTIPLETS. A. AMINO ACID ALPHABET. 1. Total number of amino acid multiplets: 6 (Expected range: 0-- 14) 2. Histogram of spacings between consecutive amino acid multiplets: (1-5) 5 (6-10) 1 (11-20) 0 (>=21) 1 3. Clusters of amino acid multiplets (cmin = 12/30 or 16/45 or 19/60): none B. CHARGE ALPHABET. 1. Total number of charge multiplets: 1 (Expected range: 0-- 6) 1 +plets (f+: 14.8%), 0 -plets (f-: 4.9%) Total number of charge altplets: 1 (Critical number: 6) 2. Histogram of spacings between consecutive charge multiplets: (1-5) 0 (6-10) 0 (11-20) 0 (>=21) 2 -------------------------------------------------------------------------------- PERIODICITY ANALYSIS. A. AMINO ACID ALPHABET (core: 4; !-core: 4) Location Period Element Copies Core Errors There are no periodicities of the prescribed length. B. CHARGE ALPHABET ({+= KR; -= ED; 0}; core: 5; !-core: 5) and HYDROPHOBICITY ALPHABET ({*= KRED; i= LVIF; 0}; core: 6; !-core: 8) Location Period Element Copies Core Errors There are no periodicities of the prescribed length. -------------------------------------------------------------------------------- SPACING ANALYSIS. Not evaluated (sequence length < 100 aa, too short).