hw6-1

240 base pairs
              MaeII                   NdeIIDpnI  FauI       ScrFI HapII
                                     Sau3AI Bsp143IMspA1I  BspMI HpaII
                                BsaMI    DpnIINspBII            MspI
AAAACCATCAAATACGTTATGCTGGAATGCAACGAACTGATCCCGCTGTTCTACGAAACCTGCCCGGCTG  Base pairs
TTTTGGTAGTTTATGCAATACGACCTTACGTTGCTTGACTAGGGCGACAAGATGCTTTGGACGGGCCGAC  1 to 70
                               BsmI     Kzo9IAclWI            MspR9I
                              Mva1269I MboIAlwI   AciI       NciI

                             Hsp92II                                MaeII  
   HphI                    NlaIII                                 SfaNI 
                                                                   TaqI
GTGAAAACATCTGCTACGAAATGTTCATGGTTGCTACCCCGAAAGTTCCGTGCGAACGTGGTTGCATCGA  Base pairs
CACTTTTGTAGACGATGCTTTACAAGTACCAACGATGGGGCTTTCAAGGCACGCTTGCACCAACGTAGCT  71 to 140
                                                                  TthHB8I 
                                                      MaeII         

         MspR9IBcnI   DpnIIMseI    ItaI         BsaOI    Bse1IHinP1I
        NciIBsiSI    Kzo9ITru1I        BbvI     Bsh1285I BseNIHspAI 
       ScrFI HapIIMboII       MboITru9I    MaeII Bst71I   BsiEIBsrI
CGTTTGCCCGGAATCTTCTCTGATCGTTAAATACGTTTGCTGCAACACCGACCGTTGCCAGTAATCCAGC  Base pairs
GCAAACGGGCCTTAGAAGAGACTAGCAATTTATGCAAACGACGTTGTGGCTGGCAACGGTCATTAGGTCG  141 to 210
           HpaIIHinfINdeIIDpnI       BsoFI    BstMCI   BsrSI   
          MspITfiI   Sau3AI Bsp143I   Fsp4HI                  

    BstH2IMboITthHB8I     BsoFI                                   
   HaeII NdeIIDpnI  Fsp4HI                                        
  AspLEISau3AI  Bsp143IItaI                                       
GCCTGATCTCTCGAAATAAAAGCCGCATTG  Base pairs
CGGACTAGAGAGCTTTATTTTCGGCGTAAC  211 to 240
 CfoIAlwNIDpnII     CviJI                                        
HhaI Bsp143IIKzo9ITaqIAciI                                       

 Enzyme		    Positions of Recognition Sites		Recognition Sequence
________	______________________________________		________________
AciI    	46 236                                		ccgc
AclWI   	43                                    		ggatc
AlwI    	43                                    		ggatc
AlwNI   	212                                   		cagnnn/ctg
AspLEI  	211                                   		gcg/c
BbvI    	183                                   		gcagc
BcnI    	64 148                                		cc/sgg
BsaMI   	30                                    		gaatgc
BsaOI   	192                                   		cgry/cg
Bse1I   	202                                   		actgg
BseNI   	202                                   		actgg
Bsh1285I	192                                   		cgry/cg
BsiEI   	192                                   		cgry/cg
BsiSI   	64 148                                		c/cgg
BsmI    	30                                    		gaatgc
BsoFI   	180 233                               		gc/ngc
Bsp143I 	38 161 214                            		/gatc
Bsp143II	212                                   		rgcgc/y
BspMI   	63                                    		acctgc
BsrI    	202                                   		actgg
BsrSI   	202                                   		actgg
Bst71I  	183                                   		gcagc
BstH2I  	212                                   		rgcgc/y
BstMCI  	192                                   		cgry/cg
CfoI    	211                                   		gcg/c
CviJI   	67 232                                		rg/cy
DpnI    	40 163 216                            		ga/tc
DpnII   	38 161 214                            		/gatc
FauI    	46                                    		cccgc
Fsp4HI  	180 233                               		gc/ngc
HaeII   	212                                   		rgcgc/y
HapII   	64 148                                		c/cgg
HhaI    	211                                   		gcg/c
Hin6I   	209                                   		g/cgc
HinP1I  	209                                   		g/cgc
HinfI   	151                                   		g/antc
HpaII   	64 148                                		c/cgg
HphI    	74                                    		ggtga
Hsp92II 	99                                    		catg/
HspAI   	209                                   		g/cgc
ItaI    	180 233                               		gc/ngc
Kzo9I   	38 161 214                            		/gatc
MaeII   	14 126 140 173                        		a/cgt
MboI    	38 161 214                            		/gatc
MboII   	158                                   		gaaga
MseI    	167                                   		t/taa
MspA1I  	45                                    		cmg/ckg
MspI    	64 148                                		c/cgg
MspR9I  	64 148                                		cc/ngg
Mva1269I	30                                    		gaatgc
NciI    	64 148                                		cc/sgg
NdeII   	38 161 214                            		/gatc
NlaIII  	99                                    		catg/
NspBII  	45                                    		cmg/ckg
Sau3AI  	38 161 214                            		/gatc
ScrFI   	64 148                                		cc/ngg
SfaNI   	138                                   		gcatc

 Enzyme		    Positions of Recognition Sites		Recognition Sequence
________	______________________________________		________________
TaqI    	137 221                               		t/cga
TfiI    	151                                   		g/awtc
Tru1I   	167                                   		t/taa
Tru9I   	167                                   		t/taa
TthHB8I 	137 221                               		t/cga

The following endonucleases are in the database, but do not cut this sequence:
AccBSI, Alw26I, BbsI, Bbv16II, BcgI, BpiI, BpmI, BpuAI, BsaI, BseRI, BsgI, 
BsiI, BsmAI, BsmBI, BsmFI, BsrBI, BsrDI, BssSI, BstD102I, BstF5I, Eam1104I, 
EarI, Eco31I, Eco57I, Esp3I, FokI, GsuI, HgaI, Ksp632I, MnlI, PleI, 
SapI, TspRI, AatI, AatII, Acc113I, Acc16I, Acc65I, AccB1I, AccB7I, AccI, 
AccII, AccIII, AclNI, AcsI, AcyI, AfaI, AfeI, AflII, AflIII, AgeI, 
AhdI, AluI, Alw21I, Alw44I, Ama87I, AocI, Aor51HI, ApaI, ApaLI, ApoI, 
AscI, AseI, AsnI, Asp700I, Asp718I, AspEI, AspHI, AspI, AspS9I, AsuI, 
AtsI, AvaI, AvaII, AviII, AvrII, BalI, BamHI, BanI, BanII, BanIII, 
BbeI, BbiII, BbrPI, BbuI, Bbv12I, BclI, BcoI, BfaI, BfrI, BglI, 
BglII, BlnI, BlpI, Bme18I, BmyI, Bpu1102I, Bpu14I, Bsa29I, BsaAI, BsaBI, 
BsaHI, BsaJI, BsaWI, Bsc4I, BscI, Bse118I, Bse21I, Bse8I, BseAI, BseCI, 
BseDI, BsePI, Bsh1236I, Bsh1365I, BshNI, BsiHKAI, BsiMI, BsiWI, BsiYI, BslI, 
BsoBI, Bsp106I, Bsp119I, Bsp120I, Bsp1286I, Bsp13I, Bsp1407I, Bsp1720I, Bsp19I, Bsp68I, 
BspCI, BspDI, BspEI, BspHI, BspLU11I, BspTI, BspXI, BsrBRI, BsrFI, BsrGI, 
BssAI, BssHII, BssT1I, Bst1107I, Bst2UI, Bst98I, BstBI, BstDEI, BstDSI, BstEII, 
BstI, BstNI, BstOI, BstPI, BstSFI, BstSNI, BstUI, BstX2I, BstXI, BstYI, 
BstZI, Bsu15I, Bsu36I, BsuRI, Cac8I, CciNI, CelII, Cfr10I, Cfr13I, Cfr42I, 
Cfr9I, CfrI, ClaI, CpoI, Csp45I, Csp6I, CspI, CvnI, DdeI, DraI, 
DraII, DraIII, DrdI, DsaI, EaeI, EagI, Eam1105I, Ecl136II, EclHKI, EclXI, 
Eco105I, Eco130I, Eco147I, Eco24I, Eco255I, Eco32I, Eco47I, Eco47III, Eco52I, Eco64I, 
Eco72I, Eco81I, Eco88I, Eco91I, EcoICRI, EcoNI, EcoO109I, EcoO65I, EcoRI, EcoRII, 
EcoRV, EcoT14I, EcoT22I, EheI, ErhI, Esp1396I, FauNDI, FbaI, FriOI, FseI, 
FspI, HaeIII, HgiEI, Hin1I, HincII, HindII, HindIII, HpaI, Hsp92I, KasI, 
Kpn2I, KpnI, Ksp22I, KspI, LspI, MaeI, MaeIII, MamI, MfeI, MflI, 
MluI, MluNI, Mph1103I, MroI, MroNI, MscI, MslI, Msp17I, MspCI, MunI, 
MvaI, MvnI, MwoI, NaeI, NarI, NcoI, NdeI, NgoAIV, NgoMI, NheI, 
NlaIV, NotI, NruI, NsiI, NspI, NspV, PacI, PaeI, PaeR7I, PalI, 
Pfl23II, PflMI, PinAI, Ple19I, PmaCI, Pme55I, PmeI, PmlI, Ppu10I, PpuMI, 
PshAI, PshBI, Psp124BI, Psp1406I, Psp5II, PspAI, PspALI, PspEI, PspLI, PspN4I, 
PspOMI, PstI, PstNHI, PvuI, PvuII, RcaI, RsaI, RsrII, SacI, SacII, 
SalI, Sau96I, SbfI, ScaI, SduI, SexAI, SfcI, SfiI, Sfr274I, Sfr303I, 
SfuI, SgfI, SgrAI, SinI, SmaI, SmiI, SnaBI, SpeI, SphI, SplI, 
SrfI, Sse8387I, Sse9I, SseBI, SspBI, SspI, SstI, SstII, StuI, StyI, 
SunI, SwaI, ThaI, Tsp45I, Tsp509I, TspEI, Tth111I, Van91I, Vha464I, VneI, 
VspI, XbaI, XcmI, XhoI, XhoII, XmaI, XmaIII, XmnI, Zsp2I