The green fluorescent protein
(GFP) from the jellyfish Aequorea victoria is rapidly
becoming an important reporter molecule for monitoring gene
expression and protein localization in vivo, in situ and in real
time. GFP emits bright green light (lambda max = 509 nm) when
excited with UV or blue light (l max = 395 nm, minor peak at
470 nm). The fluorescence excitation and emission spectra of
GFP
are similar to those of fluorescein, and the conditions used to
visualize this fluorophore are also suitable for GFP. Unlike other
bioluminescent reporters, the chromophore in GFP is intrinsic
to the primary structure of the protein, and GFP fluorescence
does not require a substrate or cofactor. GFP fluorescence is stable,
species-independent and can be monitored non-invasively in living
cells and, in the case of transparent organisms, whole
animals.
How many references can you find about
green fluorescent
protein (GFP) within five years
?
How many papers were done by P. Kitts
?
How many of these references are related to
structure ?
Can you find a paper describe its
crystal structure ?
Give its abstract
on your homepage.