HOMEWORK#5
Protein Data Bank
- 1.
Please find the 1.9 Å crystal structure of the
Aequorea victoria green
fluorescent protein (GFP) from PDB. Give the
following informations:
- a.) PDB ID number.
- b.) Name of the authors.
- c.) Expression system used by
authors.
- d.) Residue number.
- e.) Number of helix.
- f.) The starting residue and ending residue
of each helix.
- g.) Number of b-sheet.
Ans: [click here]
- 2. Show
three different Rasmol pictures of GFP:
- whatever you like.
- highlight the chromophore by spacefill and
green.
- a rotating molecule by GIF builder.
Ans: [click here]
- 3.
A blue emission variant of GFP has
been made by mutation of two residues. Please find the structure
of this blue
emission variant. Show its
structure and color the two mutated positions blue.
Ans: [click here]
PC Lyu