hk7

Homework 7

Jason Lee, a student in the department of Life Sciences,
got the mRNA from a plant.
Here is the sequence:

        1 atccactact tcatcataaa cctcacaact actattctat cttctcttct ctaattttca

       61 taatcattaa gaatggaaat ggttaacaag attgcatgct ttgtgctttt atgcatggta

      121 gtggttgcac cccatgcaga ggcactaact tgtggtcaag ttacatctac cttggctcct

      181 tgtctccctt atctaatgaa tcgcggtcct ctcggaggct gttgtggtgg tgttaagggt

      241 cttttgggtc aagcccagac tacagtagac cgacagaccg catgcacttg cctaaaatca

      301 gctgcttctt cttttacagg ccttgatttg ggcaaagctg ctagtctccc tagcacttgt

      361 agtgtcaaca tcccttacaa gatcagcccc tctactgact gctctaaagt tcagtaaagc

      421 tgatcatcag aatttggttt catgaggaga attaagaata agatagatag cattgatctt

      481 gcttatggat cctttctttc tatgttgtat cagttgtcac tttctgtttt ttctgtgttt

      541 cctttaaatt ctcgtatgta gtcgagtctt gtatcgaaat ttgacgattg attatattgt

      601 atcagttgtt actttctgtt ttcctgtgtt tcttttaaaa tcgtatgtag tcgagtcttg

      661 tatcgaaatt tcccgattgg ctatgttgta ttaatctaat ctttgataat acacatctat

      721 cttatttggt

1. Please help him to translate the DNA sequence to protein sequence.

   I H Y F I I N L T T T I L S S L L Stop F S Stop S L R Met E Met V N K I A
   C F V L L C Met V V V A P H A E A L T C G Q V T S T L A P C L P Y L Met N
   R G P L G G C C G G V K G L L G Q A Q T T V D R Q T A C T C L K S A A S S
   F T G L D L G K A A S L P S T C S V N I P Y K I S P S T D C S K V Q StopS
   StopS S E F G F Met R R I K N K I D S I D L A Y G S F L S Met L Y Q L S L
   S V F S V F P L N S R Met Stop S S L V S K F D D Stop L Y C I S C Y F L F
   S C V S F K I V C S R V L Y R N F P I G Y V V L I Stop S L I I H I Y L I W

2. Please help him to identify the complete cds of this gene.
    Use the graphic view to explain all the features of this gene.
     cds           1 AUGGAAAUGG UUAACAAAAU CGCUUGCUUC GUUCUGCUGU GCAUGGUUGU
     51 UGUUGCUCCG CACGCUGAAG CUCUGACCUG CGGUCAGGUU ACCUCCACCC
    101 UGGCUCCGUG CCUGCCGUAC CUGAUGAACC GUGGUCCGCU GGGUGGUUGC
    151 UGCGGUGGUG UUAAAGGUCU GCUGGGUCAG GCUCAGACCA CCGUUGACCG
    201 UCAGACCGCU UGCACCUGCC UGAAAUCCGC UGCUUCCUCC UUCACCGGUC
    251 UGGACCUGGG UAAAGCUGCU UCCCUGCCGU CCACCUGCUC CGUUAACAUC

       301 CCGUACAAAA UCUCCCCGUC CACCGACUGC UCCAAAGUUC AG
        graphic view

3.After translation of this gene, please help him to do the protein
   sequence analysis (including pI, mol. wt., secondary structure
   prediction, hydrophobic profile, homology search, prosite
   scanning..........)
    Molecular weight: 7352.4
    Theoretical pI: 8.88
    secondary structure prediction,hydrophobic profile
    homology search
    prosite scan