Homework 5

1. Find the following data from its header file:

PDB ID: 1BE2
Experimental metho: NMR, 10 Structures
Organism: Hordeum Vulgare
Four helixs: Gly4-Gly19, Glu26-Gln37, Ser41-Arg56, Leu63-Cys73
Four disulfide bonds: Cys3-Cys50, Cys13-Cys27, Cys28-Cys73, Cys48-Cys87
The chemical formula of the ligand: C₁₆H₃₂O₂

2. Show two different views of this protein by Rasmol, one of them must show its ligand in spacefill display.

3. Show protein in cartoon with its four disulfide bonds in yellow color.

4. Measure the bond length of each disulfide bond ( -S-S- ) and calculate the average bond length of disulfide bond.

5. Show residues in protein that are within 3.0 angstrom of carboxylic group (COO-) of ligand.
Cys13, Leu14, Val17, Try16, Ile81, Ile 69