1. Find the following data from its header file:
PDB ID 1BE2
Experimental method NMR, 10 Structures
Organism (give scientific name) and organ where this protein come from
Hordeum Vulgare
Seeds
Location of its four helixs (i.e. Ala12 - Gly20)
GLY4 - GLY19 GLU26 - GLN37 SER41
- ARG56 LEU63 - CYS73
Positions of its four disulfide bonds ( i.e. Cys3 - Cys24)
CYS3-CYS50 CYS13-CYS27
CYS28-CYS73 CYS48-CYS87
Give the chemical formula of the ligand
C16 H32 O2
2. Show two different views of this protein
by Rasmol, one of them must show its ligand in
spacefill display.
3. Show protein in cartoon with its four disulfide
bonds in yellow color.
4. Measure the bond length of each disulfide
bond ( -S-S- ) and calculate the average bond length
of disulfide bond.
the average bond length=2.01angstrom
5. Show residues in protein that are within
3.0 angstrom of carboxylic group (COO-) of ligand.
CYS, LEU, VAL, TRY, ILE, ILE