Please find the complex structure of barley lipid transfer protein with palmitate from PDB.
 
 

1. Find the following data from its header file:
(1)PDB ID
 Ans: 1BE2

(2)Experimental method
 Ans: NMR

(3)Organism (give scientific name) and organ where this protein come from
 Ans: Organism_scientific: Hordeum Vulgare
         Organ: Seeds

(4)Location of its four helixs (i.e. Ala12 - Gly20)
 Ans: Helix 1 ( Gly  4 - Gly 19 )
         Helix 2 ( Glu 26 - Gln 37 )
         Helix 3 ( Ser 41 - Arg 56 )
         Helix 4 ( Leu 63 - Cys 73 )

(5)Positions of its four disulfide bonds ( i.e. Cys3 - Cys24)
 Ans: ssbond 1 ( Cys  3 - Cys 50 )
  ssbond 2 ( Cys 13 - Cys 27 )
  ssbond 3 ( Cys 28 - Cys 73 )
  ssbond 4 ( Cys 48 - Cys 87 )

(6)Give the chemical formula of the ligand
 Ans: Formul   2  PLM    C16 H32 O2

 

2. Show two different views of this protein by Rasmol, one of them must show its ligand in spacefill display.
 Ans:

3. Show protein in cartoon with its four disulfide bonds in yellow color.
 Ans:

 

4. Measure the bond length of each disulfide bond ( -S-S- ) and calculate the average bond length of disulfide bond.
 Ans:

 

     ( 5.67 + 5.62 + 6.32 + 4.39 ) / 4 = 5.5 angstrom
5. Show residues in protein that are within 3.0 angstrom of carboxylic group (COO^-) of ligand.
 Ans: