Ans:>gi|1519357|gb|AAB07487.1| lipid transfer protein 2
MEMVNKIACFVLLCMVVVAPHAEALTCGQVTSTLAPCLPY
LMNRGPLGGCCGGVKGLLGQAQTTVDRQTACTCLKSAAS
SFTGLDLGKAASLPSTCSVNIPYKISPSTDCSKVQ
2. Please help him to identify the complete
cds of this gene.
Ans:Lycopersicon
pennellii lipid transfer protein
1 gtaatccagc taagaacgtc agaagtaaaa caaacttgtc gtaaaatatt taatttgaag
61 ttgtatttaa atcttaatta ttttttttta aagctatact cacatcattt caattattct
121 ttttgtaaaa gtatctctag agcttcataa tttttttttt aaaaatcttc gatcaaactg
181 ttagagtagg taaaagtctc acattgatgg ggaaatagac tgattatttg cttataagga
241 tgtggacaat actcctctca tataatagca tttaagatta aattagacct aaataacata
301 ttttagcatg atattagagt tatattcatt cttgtttgaa cttccgatcc acatctcaat
361 tggatctaca taaaaaaggg atattaaagt aagtaaaagc cctacattaa tcgaggaatc
421 tacttatacg aactttggtg ataaaaaaaa agactcctac acgtaagatg ttagaactag
481 ctaccacatg actttagagc cagcataata atgtacacca tcaaaatgct ttaaattttc
541 aacctaacaa ccaactacct ctctcactcc tccattggcc atctactcca aatttccctc
601 tataaaaaca ctcaaccaaa acacatttct tctcatccac tacttcatca taaacctcac
661 aactactatt ctatcttctc ttctctaatt ttcataatca ttaagaatgg aaatggttaa
721 caagattgca tgctttgtgc ttttatgcat ggtagtggtt gcaccccatg cagaggcact
781 aacttgtggt caagttacat ctaccttggc tccttgtctc ccttatctaa tgaatcgcgg
841 tcctctcgga ggctgttgtg gtggtgttaa gggtcttttg ggtcaagccc agactacagt
901 agaccgacag accgcatgca cttgcctaaa atcagctgct tcttctttta caggccttga
961 tttgggcaaa gctgctagtc tccctagcac ttgtagtgtc aacatccctt acaagatcag
1021 cccctctact
gactgctcta agtatgttaa tttttcatct tttttgacct ataacaacac
1081 ctaactcttc
gtattaatcc tagtacgaaa aataaagtaa caaaaaaatg atatgtgcta
1141 gcacattgtc
acaatatgac atgcaagtgt gtttggtttt ctcaaaaaat aagtggattt
1201 tttatttata
ttttagtgtt aagaaatatt agtttaaaaa tatttatata tgtaattata
1261 aagaaaaaag
atactattat agttagtaca ttatgttttt gttatcatta tcattattat
1321 tattattaat
gttggttttg ttcattgtta atgcagagtt cagtaaagct gatcatcaga
1381 atttggtttc
atgaggagaa ttaagaataa gatagatagc attgatcttg cttatggatc
1441 ctttctttct
atgttgtatc agttgtcact ttctgttttt tctgtgtttc ctttaaattc
1501 tcgtatgtag
tcgagtcttg tatcgaaatt tgacgattga ttatattgta tcagttgtta
1561 ctttctgttt
tcctgtgttt cttttaaaat cgtatgtagt cgagtcttgt atcgaaattt
1621 cccgattggc
tatgttgtat taatctaatc tttgataata cacatctatc ttatttggta
1681 tatgtactct
ctcgtctatt caatattttt ggtctacttt tactagggtt tttttaatat
1741 gcattacaca
tatatatcaa attcgagtaa tatatagtat acgctattgt gtgctcattc
1801 atctaggtac
ctcctttttc taaccacttc ttacacgtac aatgctaatt attg
Use the graphic
view to explain all the features of this gene.
Ans:graphic
view-1
graphic
view-2
3.After translation of this gene, please help him to do
the protein sequence analysis
(including pI, mol. wt., secondary
structure prediction, hydrophobic profile,homology search, prosite scanning..........)
Ans:
| Number of amino acids | 114 |
| Molecular weight | 11715.8 |
| Theoretical pI | 8.36 |
| Amino acid composition | linkage |
| Atomic composition | linkage |
| molecular weight | linkage |
| hydrophobic profile | linkage |
| secondary structure prediction | alpha-helix |
| beta-sheet | |
| homology search | linkage |
| prosite scanning | linkage |