5th Homework
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The data of barley lipid transfer protein with palmitate:
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Its PDB ID is 1BE2.
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The experimental method is NMR, 10 Structures.
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The organism where this protein come from is seeds of Hordeum vulgare.
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The location of its four helixs are helix 1 (GLY4 - GLY19), helix 2 (GLU26
- GLN37), helix 3 (SER41 - ARG56) and helix 4 (LEU63 - CYS73).
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The positions of its four disulfide bonds are ssbond 1 (CYS3 - CYS50),
ssbond 2 (CYS13 - CYS27), ssbond 3 (CYS28 - CYS73) and ssbond 4 (CYS48
- CYS87).
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The chemical formula of the ligand is C16H32O2.
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The two different views of this protein by Rasmol:
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The protein is shown in cartoon with its four disulfide bonds in yellow color:
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The bond length of each disulfide bond and the average bond length are Cys3 -> Cys50: 5.67 angstrom, Cys13 -> Cys27: 6.32 angstrom, Cys28 -> Cys73: 4.39 angstrom, and Cys 48 -> Cys 87: 5.63 angstrom, and the average bond length is 5.50 angstrom.
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The residues in protein that are within 3.0 angstrom of carboxylic
group (COO-) of ligand are CYS 13, LEU 14, VAL 17, TRY 16, ILE81, ILE 69 .
