HOMEWORK#5

• due on 12/7

Please find the complex structure of barley lipid transfer protein with palmitate from PDB.
 
 

1. Find the following data from its header file:

• PDB ID : 1BE2
• Experimental method : NMR, 10 structures
• Organism (give scientific name) and organ where this protein come from
•   LIPID Transport , organism (Hordeum Vulgare)
• Location of its four helixs (i.e. Ala12 - Gly20)
• Positions of its four disulfide bonds ( i.e. Cys3 - Cys24)
• Give the chemical formula of the ligand
• Mol_id

 

2. Show two different views of this protein by Rasmol, one of them must show its ligand in spacefill display.

3. Show protein in cartoon with its four disulfide bonds in yellow color.

4. Measure the bond length of each disulfide bond ( -S-S- ) and calculate the average bond length of disulfide bond.

5. Show residues in protein that are within 3.0 angstrom of carboxylic group (COO^-) of ligand.
 
 

　
 
 

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PCL 11/25/99