Question:
Open Reading Frame of MLH1
| Frame | from | | to | Length |
| +1 | 22 | .. | 2292 | 2271 |
| -1 | 1396 | .. | 1650 | 255 |
| +2 | 926 | .. | 1120 | 195 |
| +2 | 1304 | .. | 1492 | 189 |
| -2 | 489 | .. | 677 | 189 |
| -2 | 1353 | .. | 1523 | 171 |
| +2 | 2231 | .. | 2368 | 138 |
| -2 | 1716 | .. | 1853 | 138 |
| -2 | 2358 | .. | 2483 | 126 |
| -2 | 1 | .. | 119 | 119 |
| -2 | 2070 | .. | 2177 | 108 |
| +3 | 3 | .. | 110 | 108 |
Open Reading Frame mutS
|
| Frame | from | | to | Length |
| +1 | <1 | .. | 2561 | 2562 |
| -1 | 565 | .. | 936 | 372 |
| -2 | 432 | .. | 803 | 372 |
| +2 | 2243 | .. | 2548 | 306 |
| -1 | 1177 | .. | 1449 | 273 |
| -1 | 1546 | .. | 1755 | 210 |
| -2 | 1029 | .. | 1235 | 207 |
| +2 | 728 | .. | 922 | 195 |
| -2 | 1698 | .. | 1850 | 153 |
| -1 | 1 | .. | 147 | 147 |
| +2 | 1583 | .. | 1696 | 114 |
| +2 | 362 | .. | 472 | 111 |
| -2 | <255 | .. | 356 | 102 |
| +3 | 3 | .. | 104 | 102 |
1.
No significant alignment
2.
protien sequence of mutS
protien sequence of MLH1
3.
GI:13878583, MLH1_Mouse, DNA MISMATCH REPAIR PROTEIN MLH1 (MUTL PROTEIN HOMOLOG 1).
GI:13591989, NP_112315, mismatch repair protein [Rattus norvegicus].
GI:4557757, NP_000240, mutL homolog 1; mutL (E. coli) homolog 1 [Homo sapiens].
GI:466462, AAA17374, human homolog of E. coli mutL gene product
GI:604369, AAA85687.1, hMLH1 gene product.
GI:12835158, BAB23172.1, putative [Mus musculus].
GI:13543339, AAH05833.1, Similar to mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2) [Homo sapiens].
GI:7304079, AAF59117.1, Mlh1 gene product [Drosophila melanogaster].
GI:3192877, AAC19117.1, mutL homolog [Drosophila melanogaster].
GI:460627, AAA16835.1, Mlh1p.
4.
| Mus musculus | Identities = 651/760 (85%), Positives = 693/760 (90%), Gaps = 4/760 (0%) | pairwise alignment |
| Rattus norvegicus | Identities = 639/758 (84%), Positives = 684/758 (89%), Gaps = 3/758 (0%) | pairwise alignment |
| Drosophila melanogaster | Identities = 335/751 (44%), Positives = 453/751 (59%), Gaps = 94/751 (12%) | pairwise alignment |
5.
conserve domain (CD) of MLH1
| position: | No.147-327 amino acid |
| name: | DNA_mis_repair, DNA mismatch repair protein. Also known as the mutL/hexB/PMS1 pfam family. |
| ID: | pfam01119 |
6.
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|
consensus 1 GTTVEVRDLFYNLPVRRKFLKSPKKEFRKILDLLQRYALIHPNVSFSLTKEG--KALLQL 58
query 147 GTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQG--ETVADV 204
1B63_A 144 GTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNG--KIVRQY 201
gi 8039787 159 GTVVRVEQLFENFPARKRFLGRQSAETTLCRSALIDVSLAHHPVEFRFTVDGthKLTLLS 218
gi 8928214 141 GTIVDVTKIFHNFPARKRFLKQEPIETKMCLKVLEEKIITHPEINFEIN-LN--QKLRKI 197
gi 3914081 141 GTEVEVRDLFFNLPVRRKFLKKEDTERRKVLELIKEYALTNPEVEFTLFSEG--RETLKL 198
gi 3914082 141 GTEVEVYDLFFNLPARKKFLRKEDTERRKITELVKEYAITNPQVDFHLFSEG--KETLNL 198
70 80 90 100 110 120
....*....|....*....|....*....|....*....|....*....|....*....|
consensus 59 KTSP--S-SLKERIRSVFGTAVLKNLIPF--EEKDGDFRIEG-FISSPNVSR-SSRDRQF 111
query 205 RTLP--NaSTVDNIRSIFGNAVSRELIEIgcEDKTLAFKMNG-YISNANYSV--KKCIFL 259
1B63_A 202 RAVPegG-QKERRLGAICGTAFLEQALAI--EWQHGDLTLRG-WVADPNHTTpALAEIQY 257
gi 8039787 219 QQTR--K-DRCLETQMLKGDPALFHTIEG--G--DCSFHFHLvLSEPAICRR--ERRGIF 269
gi 8928214 198 YFK---E-SLIDRVQNVYGNVIENNKFRV--LKKEHDNIKIEiFLAPDNFSK-KSKRHIK 250
gi 3914081 199 KKS-----SLKERVEEVFQTKTEELYAER--E--GITLRA---FVSRNQRQG-----KYY 241
gi 3914082 199 KKK-----DLKGRIEEIFESIFEEESSER--E--GIKVRA---FISRNQKRG-----KYY 241
130 140 150 160 170 180
....*....|....*....|....*....|....*....|....*....|....*....|
consensus 112 LFINGRPVEDKLLLKAIREVYATYLPRGRYPVFVLNLELPPELVDVNVHPDKKEVRLLKE 171
query 260 LFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHE 319
1B63_A 258 CYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQS 317
gi 8039787 270 TFVNGRRIFDYGLVQALVLGSEGYFPNGTFPVACLFLTVNSERIDFNIHPAKKEVHLQDY 329
gi 8928214 251 TFVNRRPIDQKDLLEAITNGHSRILSPGNFPICYLFLEINPEYIDFNVHPQKKEVRFYNL 310
gi 3914081 242 VFINKRPIQNKNLKEFLRKVFG------YKTLVVLYAELPPFMVDFNVHPKKKEVNILKE 295
gi 3914082 242 LFVNSRPVYNKNLKEYLKKTFG------YKTIVVLFIDIPPFLVDFNVHPKKKEVKFLKE 295
....*...
consensus 172 EEILDLIK 179
query 320 ESILERVQ 327
1B63_A 318 RLVHDFIY 325
gi 8039787 330 AHIRHTLS 337
gi 8928214 311 PFLFKLIS 318
gi 3914081 296 RKFLELVR 303
gi 3914082 296 RKIYELIR 303
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