Institute of Molecular and Cellular Biology

Chien-Chung Chang, Ph.D.

Assistant Professor

Laboratory of Molecular Immunobiology

Institute of Molecular and Cellular Biology

National Tsing Hua University

101 Sec. 2 Kuang Fu Road

Hsinchu, Taiwan 30013

 

Phone: 03-574-2475

Fax: 03-571-5934

E-Mail: ccchang@life.nthu.edu.tw

 

Current research interests

Publications

Articles

International conference proceedings

Patent

Why do tumors grow in spite of a competent host immune system? Do cancer cells develop strategies to evade host tumor-specific immune responses? How are the gene products of MHC, or major histocompatibility complex, and components of antigen processing machinery manipulated by malignant cells? How does intrinsic genotoxic stress contribute to NKG2D-related innate self/non-self discrimination imbalance, which promotes cancer immune surveillance and escape? These interesting and fundamental questions are addressed at the molecular level in the Chang Laboratory using a number of state-of-the-art cellular, molecular biological, biochemical and structural biological approaches.   

Hsieh, C.-H., Hsu, Y.-J., Chang, C.-C., Liu, H.-C., Chuang, K.-L., Chuang, C.-K., Pang, S.-T., Hasumi, K., Ferrone, S. and Liao, S.-K. (2009) Total HLA class I loss in a sarcomatoid renal cell carcinoma cell line caused by the coexistence of distinct mutations in the two encoding β₂-microglobulin gene. Cancer Immunol. Immunother. 58:395-408.

 

Wongsena, W., Sconocchia, G., Cho, H.-S., Chang, C.-C., Wang, X., Klumkrathok, K., Ferrone, S., and Leelayuwat, C. (2008) Production and characterization of monoclonal antibodies against major histocompatibility complex class I chain-related gene A. Tissue Antigens. 72:431-40.

 

Chang, C.-C., Pirozzi, G., Errico, S., Luongo, M., Lombardi, M.L., and Ferrone, S. (2008) Combination of germline and somatic TAPBP gene mutations, HLA haplotype loss and epigenetic silencing of HLA-A gene responsiveness to IFN-γ in melanoma cells with HLA class I downregulation. In revision.

 

Sangrouber, D, Marcou, C., Le Discorde, M., Chang, C.-C., Carosella, E.D. and Moreau, P. (2007) Cellular co-localization of intron 4–containing mRNA and HLA-G soluble protein in melanoma analyzed by fluorescence in situ hybridization. J. Immunol. Methods. 326: 54-62.

 

Chang, C.-C. and Ferrone, S. (2007) Immune selective pressure and HLA class I antigen defects in malignant lesions. Cancer Immunol. Immunother. 56: 227-236.

 

Chang, C.-C. and Ferrone, S. (2006) NK cell activating ligands on human malignant cells: molecular and functional defects and potential clinical relevance. Semin. Cancer Biol. 16: 383-392.

 

Chang, C.-C., Ogino, T, Mullin, D.W., Yamshchikov, G.V., Bandoh N., Slingluff, C.L. Jr., and Ferrone, S. (2006) Defective HLA class I-associated antigen presentation caused by a novel β2-microglobulin loss-of-function in melanoma cells. J. Biol. Chem. 281: 18763-18773.

 

Shen, C., Chang, C.-C., Zhang, J., Guo, W., Xia, L., Meng, F., and Xie, W. (2006) Structural and functional characterization of peptide-β2m fused HLA-A2/MART127-35 complexes. Biochem. Biophys. Res. Commun. 342: 57-65.

 

Chang, C.-C., Hernandez-Guzman, F.G., Luo, W., Wang, X., Ferrone, S., and Ghosh, D. (2005) Structural basis of antigen mimicry in a clinically relevant melanoma antigen system. J. Biol. Chem. 280: 41546-41552.

 

Yan, W.-H., Lin, A.-F., Chang, C.-C., and Ferrone, S. (2005) Induction of HLA-G expression in a melanoma cell line OCM-1A following the treatment with 5-aza-2'-deoxycytidine. Cell Res. 15: 523-531.

 

Yamshchikov, G.V., Mullins, D.W., Chang, C.-C., Ogino, T., Thompson, L., Presley, J., Galavotti, H., Aquila, W., Deacon, D., Ross, W., Patterson, J.W., Engelhard, V.H., Ferrone, S., and Slingluff, C.L., Jr. (2005) Sequential immune escape and shifting of T cell responses in a long-term survivor of melanoma. J. Immunol. 174: 6863-6871.

 

Chang, C.-C., Campoli, M., and Ferrone, S. (2005) Classical and non-classical HLA class I antigen and NK cell activating ligand changes in malignant cells: current challenges and future directions. Adv. Cancer Res. 93: 189-234.

 

Chang, C.-C., Campoli, M., Restifo, N.P., Wang, X., and Ferrone, S. (2005) Immune selection of hot-spot β2-microglobulin gene mutations, HLA-A2 allospecificity loss and antigen processing machinery component downregulation in melanoma cells derived from recurrent metastases following immunotherapy. J. Immunol. 174: 1462-1471.

 

Chang, C.-C., Campoli, M., Luo, W., Zhao, W., Zanker, K.S., and Ferrone, S. (2004) Immunotherapy of melanoma targeting human high molecular weight-melanoma associated antigen: potential role of non-immunological mechanisms. Ann. NY Acad. Sci. 1028: 340-350.

 

Campoli, M., Chang, C.-C., Kageshita, T., Wang, X., McCarthy, J.B., and Ferrone, S. (2004) Human High Molecular Weight-Melanoma Associated Antigen (HMW-MAA): a melanoma cell surface chondroitin sulfate proteoglycan (MCSP) with biological and clinical significance. Crit. Rev. Immunol. 24: 267-296.

 

Chang, C.-C., Campoli, M., and Ferrone, S. (2004) HLA class I antigen expression in malignant cells: why does it not always correlate with CTL-mediated lysis? Curr. Opin. Immunol. 16: 644-650.

 

Campoli, M., Chang, C.-C., Oldford, S.A., Edgecombe, A.D., Drover, S., and Ferrone, S. (2004) HLA antigen changes in malignant tumors of mammary epithelial origin: molecular mechanisms and clinical implications. In: Breast Disease. Immunology of Breast Cancer, Wei, W.Z., Lopez, D.M. (eds), Amsterdam: IOS Press, 20: 105-125.

 

Pirozzi, G., Chang, C.-C., Errico, S., Curci, A., Luongo, V., Paino, F., Di Gennaro, E., Ferrone, S., and Lombardi, M.L. (2004) Molecular defects underlying HLA class I antigen abnormalities in melanoma cells. In: HLA 2004:  Immunobiology of the human MHC. Hansen J., Dupont, B. (eds.), Vol. I, pp. IHWG Press, Seattle, Washington.

 

Campoli, M., Chang, C.-C., Wang, X., and Ferrone, S. (2003) HLA class I antigen processing machinery and HLA class I antigen derived peptide complex defects in tumor cell escape.  In: Cancer Immunotherapy at the crossroad – How tumors evade immunity and what can be done, Finke, J.H., Bukowski, R.M. (eds). Totowa: The Humana Press Inc., pp. 3-34.

 

Chang, C.-C., Campoli, M., and Ferrone, S. (2003) HLA class I defects in malignant lesions: what have we learned? Keio J. Med. 52: 220-229.

 

Chang, C.-C., Murphy, S.P., and Ferrone, S. (2003) Differential in vivo and in vitro HLA-G expression in melanoma cells: potential mechanisms. Hum. Immunol. 64: 1057-1063.

 

Chang, C.-C. and Ferrone, S. (2003) HLA-G in melanoma: can the current controversies be solved? Semin. Cancer Biol. 13: 361-369.

 

Campoli, M., Chang, C.-C., and Ferrone, S. (2002) HLA class I antigen loss, tumor immune escape and immune selection. Vaccine. 20 Suppl 4: A40-A45.

 

Pende, D., Rivera, P., Marcenaro, S., Chang, C.-C., Biassoni, R., Kubin, M., Cosman, D., Ferrone, S., Moretta, L., and Moretta, A. (2002) Major histocompatibility complex class I-related chain A and UL16-binding protein  expression on tumor cell lines of different histotypes: analysis of tumor susceptibility to NKG2D-dependent natural killer cell cytotoxicity. Cancer Res. 62: 6178-6186.

 

Chang, C.-C. and Ou, J.T. (2002) Excess production of interleukin-12 subunit p40 stimulated by the virulence plasmid of Salmonella enterica serovar Typhimurium in the early phase of infection in the mouse. Microb. Pathog. 32: 15-25.

 

Hor, L.-I., Chang, Y.-K., Chang, C.-C., Lei, H.-Y. and Ou, J.T. (2000) Mechanism of high susceptibility of iron-    overloaded mouse to Vibrio vulnificus infection. Microbiol. Immunol. 44: 871-878.

 

 

Chang, C.-C., Luo, W., Wang, X., Ferrone, S., and Ghosh, D. (2006) Structural basis of tumor antigen mimicry by a mimotope isolated from a phage display peptide library. J. Immunol. 176: S261. The American Association of Immunologists 2006 Annual Meeting, Boston, MA.

 

Chang, C.-C. and Ferrone, S. (2004) HER2/neu-targeted breast cancer immunotherapy: a hybrid protein approach. The Susan G. Komen Breast Cancer Foundation 2004 Mission Conference, New York, NY.

 

Yamshchikov, G.V., Thompson, L., Mullins, D.W., Ferrone, S., Ogino, T., Chang, C.-C., Galavotti, H., Aquila, W., Deacon, D., Ross, W., Patterson, J.W., Engelhard, V.H., and Slingluff, C.L., Jr. (2003) Immun escape, immune editing, and immune adaptation in a long-term survivor of metastatic melanoma. The 18th Annual Scientific Meeting of the International Society for Biological Therapy of Cancer, Bethesda, MD. J. Immunother. 26: S45, 2003

 

Chang, C.-C., Pirozzi, G., Lombardi, M.L., and Ferrone, S. (2003) Identification of a tapasin gene mutation in a human melanoma cell line with HLA class I antigen downregulation. The 90th Annual Meeting of the American Association of Immunologists, Denver, CO. FASEB J. 17: C326, 2003.

 

Bandoh N., Chang, C.-C., Cho, H.S., Wang, X, Ogino, T., Harabuchi, Y., Whiteside, T.L., and Ferrone, S. (2003) HLA class I antigen downregulation in human head and neck squamous cell carcinoma cell lines: Role of antigen processing machinery defects. The 94th Annual Meeting of the American Association for Cancer Research, Washington, D.C. Proc. Am. Assoc. Cancer Res. 44: 770, 2003.

 

Pirozzi, G., Chang, C.-C., Errico, S., Curci, A., Luongo, V., Paino, F., DiGennaro, E., Ferrone, S. and Lombardi, M.L. (2002) Molecular defects underlying HLA class I antigen abnormalities in melanoma cells. The 13th International Histocompatibility Workshop and Congress, Seattle, WA. Tissue Antigens. 59: S90, 2002.

 

Chang, C.-C., Bankert, R.B. and Ferrone, S. (2002) Multiple molecular lesions in a human lung carcinoma cell line with HLA antigen loss. The 13th International Histocompatibility Workshop and Congress, Seattle, WA. Tissue Antigens. 59: S84-S85, 2002.

 

Chang, C.-C. and Ou, J.T. (1999) Excess stimulation of p40, a subunit of interleukin 12, production by the Salmonella typhimurium virulence plasmid. The 99th General Meeting of American Society for Microbiology, Chicago, IL.

"Peptides for stimulating an immune response against melanoma", U.S. Patent Application 20070190061, Kind Code A1, approved August 16, 2007