ProtParam tool
DE LYMPHOID ENHANCER BINDING FACTOR 1.
OS MUS MUSCULUS (MOUSE).
The parameters have been computed for the following feature:
FT DNA_BIND 297 365 HMG BOX.
Considered sequence fragment:
IKKPLNAFML YMKEMRANVV AECTLKESAA INQILGRRWH ALSREEQAKY YELARKERQL
HMQLYPGWS
References and documentation are available.
Number of amino acids: 69
Molecular weight: 8239.6
Theoretical pI: 9.66
Amino acid composition:
Ala (A) 8 11.6%
Arg (R) 6 8.7%
Asn (N) 3 4.3%
Asp (D) 0 0.0%
Cys (C) 1 1.4%
Gln (Q) 4 5.8%
Glu (E) 7 10.1%
Gly (G) 2 2.9%
His (H) 2 2.9%
Ile (I) 3 4.3%
Leu (L) 8 11.6%
Lys (K) 6 8.7%
Met (M) 4 5.8%
Phe (F) 1 1.4%
Pro (P) 2 2.9%
Ser (S) 3 4.3%
Thr (T) 1 1.4%
Trp (W) 2 2.9%
Tyr (Y) 4 5.8%
Val (V) 2 2.9%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 7
Total number of positively charged residues (Arg + Lys): 12
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 16600 16800 16700 16500 16000
Abs 0.1% (=1 g/l) 2.015 2.039 2.027 2.003 1.942
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 16600 16800 16700 16500 16000
Abs 0.1% (=1 g/l) 2.015 2.039 2.027 2.003 1.942
Estimated half-life:
The N-terminal of the sequence considered is I (Ile).
The estimated half-life is: 20 hour (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hour (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 63.47
This classifies the protein as unstable.
Aliphatic index: 82.17
Grand average of hydropathicity (GRAVY): -0.584