1. Give their accession number.

    3. LEF-1: P27782; TCF-1:Q00417
     
     
  3. They share the same DNA-binding domain. What's the name of this domain? What is the residue number corresponding to the full-length protein?

    5. DNA-bind HMG box; LEF-1 397 aa 297-365, TCF-1 303 aa 188-256
  5. Show the AA sequence of this DNA-binding domain of each protein.

    7. LEF-1   KKP LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL YPGWS

    TCF-1   IKK PLNAFMLYMK EMRAKVIAEC TLKESAAINQ ILGRRWHALS REEQAKYYEL ARKERQLHMQ LYPGWS
     
     

  7. Calculate the pI/Mw and predict the secondary of this DNA-binding domain of LEF-1.

    9. Answer
     
     
  9. Calculate the hydropathicity ( Kyte & Doolittle) of this DNA-binding domain of TCF-1.

    10. SEQUENCE LENGTH: 69

    Hphob. / Kyte & Doolittle, the individual values for the 20 amino acids are: 
    Ala:  1.800  Arg: -4.500  Asn: -3.500  Asp: -3.500  Cys:  2.500  Gln: -3.500  
Glu: -3.500  Gly: -0.400  His: -3.200  Ile:  4.500  Leu:  3.800  Lys: -3.900  
Met:  1.900  Phe:  2.800  Pro: -1.600  Ser: -0.800  Thr: -0.700  Trp: -0.900  
Tyr: -1.300  Val:  4.200  Asx: -3.500  Glx: -3.500  Xaa: -0.490
 
   
   
  10. Show the result of chymotrypsin cleavage of TCF-1. ( Displaying peptides with a mass bigger than 1000 Dalton with maximum number of missed cleavages = 1 )

    12. Answer
     
     
  12. Show the result of Prosite scanning of LEF-1.

    13. Answer