ProtParam tool

User provided sequence:
MDPRKVSELR AFVKMCRQDP SVLHTEEMRF LREWVESMGG KVPPATHKAK SEENTKEEKR 
DKTTEDNIKT EEPSSEESDL EIDNEGVIEA DTDAPQEMGD ENAEITEAMM DEANEKKGAA 
IDALNDGELQ KAIDLFTDAI KLNPRLAILY AKRASVFVKL QKPNAAIRDC DRAIEINPDS 
AQPYKWRGKA HRLLGHWEEA ARDLALACKL DYDEDASAML REVQPRAQKI AEHRRKYERK 
REEREIKERI ERVKKAREEH EKAQREEEAR RQSGSQFGSF PGGFPGGMPG NFPGGMPGMG 
GAMPGMAGMP GLNEILSDPE VLAAMQDPEV MVAFQDVAQN PSNMSKYQNN PKVMNLISKL 
SAKFGGHS

References and documentation are available.

Number of amino acids: 368 Molecular weight: 41279.5 Theoretical pI: 5.28 Amino acid composition: Ala (A) 39 10.6% Arg (R) 26 7.1% Asn (N) 16 4.3% Asp (D) 24 6.5% Cys (C) 3 0.8% Gln (Q) 14 3.8% Glu (E) 45 12.2% Gly (G) 25 6.8% His (H) 7 1.9% Ile (I) 16 4.3% Leu (L) 22 6.0% Lys (K) 30 8.2% Met (M) 18 4.9% Phe (F) 10 2.7% Pro (P) 22 6.0% Ser (S) 19 5.2% Thr (T) 9 2.4% Trp (W) 3 0.8% Tyr (Y) 5 1.4% Val (V) 15 4.1% Asx (B) 0 0.0% Glx (Z) 0 0.0% Xaa (X) 0 0.0% Total number of negatively charged residues (Asp + Glu): 69 Total number of positively charged residues (Arg + Lys): 56 Extinction coefficients: Conditions: 6.0 M guanidium hydrochloride 0.02 M phosphate buffer pH 6.5 -1 -1 Extinction coefficients are in units of M cm . The first table lists values computed assuming ALL Cys residues appear as half cysteines, whereas the second table assumes that NONE do. 276 278 279 280 282 nm nm nm nm nm Ext. coefficient 23885 24181 24065 23830 23160 Abs 0.1% (=1 g/l) 0.579 0.586 0.583 0.577 0.561 276 278 279 280 282 nm nm nm nm nm Ext. coefficient 23450 23800 23705 23470 22800 Abs 0.1% (=1 g/l) 0.568 0.577 0.574 0.569 0.552 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hour (mammalian reticulocytes, in vitro). >20 hour (yeast, in vivo). >10 hour (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 59.06 This classifies the protein as unstable. Aliphatic index: 62.69 Grand average of hydropathicity (GRAVY): -0.870