**Q:
A protein with extinction coefficient E ^{1%} = 16 yields an absorbance
of 0.73 when measured in a 0.5-cm cell. Calculate the weight concentration.**

**Solution**

The absorbance in E^{1%} can be written as,

A = E

^{1%}* b * c

Where A = 0.73 in a 0.5-cm cell, and = 16. Then, the weight concentration is

c = A / (E

^{1%}* b) = 0.73 / (16*0.5) = 0.091 g/100ml