Q: A protein with extinction coefficient E1% = 16 yields an absorbance of 0.73 when measured in a 0.5-cm cell. Calculate the weight concentration.
The absorbance in E1% can be written as,
A = E1% * b * c
Where A = 0.73 in a 0.5-cm cell, and = 16. Then, the weight concentration is
c = A / (E1% * b) = 0.73 / (16*0.5) = 0.091 g/100ml