Laboratory in Molecular Biology

分子生物學實驗技術與原理

90LS 482101   Wed  13:00~17:00

90LS 482102   Thr   13:00~17:00

週數

Wed

Thr

實驗主題

報告

1

9/19

9/20

Organization, Check in and equipment introduction

 

2

9/26

9/27

Preparation of buffers and media

 

3

10/3

10/4

Inoculation of bacteria culture

 

4

10/10

10/11

10日國慶日放假一天, 11日停課

 

5

10/17

10/18

Small scale preparation of plasmid DNA

 

6

10/24

10/25

Restriction digestion of DNA & agarose gel electrophoresis

* 

7

10/1

11/1

Polymerase Chain Reactions (PCR)

 

8

11/7

11/8

Purification of cloning vector and insert

 

9

11/14

11/15

Spectroscopic analysis & construction of recombinant DNA

 

10

11/21

11/22

22日全校運動大會,停課一天,21日停課

 

11

11/28

11/29

Competent E. coli host cells and transformation

*

12

12/5

12/6

Characterization of recombinant DNA (I) 

 

13

12/12

12/13

Characterization of recombinant DNA (II) 

 

14

12/19

12/20

Protein expression

 

15

12/26

12/27

SDS-polyacrylamide gel electrophoresis

*  

16

91/1/2

91/1/3

Proposal, Final Exam, DNA sequencing and Check out

 

教師 : 張大慈(# 2767) 黃海美 (# 2479)

助教 : 林淑貞(# 5859) 柯佩宜 (# 5859)

講課教室 : LS-II-329

實驗教室 : LS-II-321

教材 : 實驗講義

成績 : 報告40%(加注*之實驗後一週內需繳交已進行之所有實驗之實驗報告, 且於第16週時另作一份proposal), 考試30%, 平時成績 30%

參考文獻 :

(1)   Molecular Biology. Weaver, R.F. 1999, WCB MaGraw-Hill,

(2)   Molecular cloning, 2nd Ed. Sambrook, J., Fritsch, E.F., Maniatis, T. 1989, Cold Spring Harbor Laboratory Press,

(3)   Recombinant DNA, 2nd Ed. Watson, J. D., Wirkowski, J., Gilman, M., Zoller, M. 1992, Scientific American Books.

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