Mos Homework 4

1. Sho-Ming cloned a gene in the lab. The DNA sequence is listed here:
AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAA
CCCTAAGGTCCTATAGTGTTCTATATCCAACACTAGCTCCTAC
TCCCTAAAGCATTTATTATATCTTCCCCTAGCTAGATACTTCA
TTCCACAAATAGTTTGCAGCTTTTTCTTTTCCTCTAAAACAAT
GGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTGCATG
GTGGTAGCTGCACCCTGCGCAGAAGCCATAACCTGTGGCCAGG
TTACGTCGAATTTGGCACCTTGTCTTGCTTATCTTAGAAACAC
vGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGTG
AATTCTGCAAGGACCACAGAAGATCGTCAAATTGCATGCACTT
GCCTGAAATCAGCTGCAGGTGCTATTTCTGGAATCAATTTGGGC
AAAGCTGCTGGTCTCCCTAGTACTTGTGGTGTCAATATTCCTTA
CAAGATCAGCCCTTCCACTGACTGCTCCAAGTACCTCACTTTTT
TTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTTCAT
TTTCGCTTATCTTTTAAATTTTTTATTCGGAATCTTTATACCA
Please help him to identify the gene and its protein sequence.

ANS


 This sequence given (605 bps) has 98% homology with bp141 to bp745 of N.tabacum ltp1 gene for lipid transferase by NCBI Blast search. And the protein sequence of lipid transferase as below: 
translation="MEIAGKIACFVVLCMVVAAPCAEAITCGQVTSNLAPCLAYLRNT
                     GPLGRCCGGVKALVNSARTTEDRQIACTCLKSAAGAISGINLGK
                     AAGLPSTCGVNIPYKISPSTDCSKVQ"
From bp310 to bp644 on ltp1 gene

2. Please find the gene that encode the trp repressor. Show its mRNA on your home page.

ANS


 The 1289 bps E.coli trpR gene coding for the trp operon repressor protein. The mRNA transcripts from DNA sequence from bp329 to bp711.
View it's mRNA

3. How many nucleotide and protein sequence of Lycopersicon esculentum were know? Please find its class II small heat shock protein mRNA, complete cds.

ANS


 There are 679 nucleotides and 832 protein sequences known by taxonomy browser. Lycopersicon esculentum's Taxonomy Id is 4081, common name is tomato.
View Lycopersicon esculentum
class II small heat shock protein
mRNA
complete cds

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