David Liu, same guy from last homework, also isolated a cDNA clone from snake

venom library. The nucleotide sequence of this cDNA clone is shown here:

AAAACCATCAAATATGTTATGCTGGAATGCAACGAACTGATCCCGCTGTTC

TACGAAACCTGCCCGGCTGGTGAAAACATCTGCTACGAAATGTTCATGGTT

GCTACCCCGAAAGTTCCGTGCGAACGTGGTTGCATCGACGTTTGCCCGGAA

TCTTCTCTGATCGTTAAATACGTTTGCTGCAACACCGACCGTTGCCAGTAAT

CCAGCGCCTGATCTCTCGAAATAAAAGCCGCATTG

(1) Please help him to find its corresponding polypeptide sequence (DNA -> Protein

translation).

MLECNELIPL FYETCPAGEN ICYEMFMVAT PKVPCERGCI DVCPESSLIV KYVCCNTDRCQ

(61 amino acids)

(2) Please help him to calculate pI/Mw of thispolypeptide, perform the trypsin cutting ,

analyze the cutting pattern and report the fragments with molecular weight over 500

Dalton.

[Theoretical pI/Mw: 4.29 / 6917.13 ]

mass position peptide sequence

---------- --------- ----------------

3699.66 1- 32 MLECNELIPLFYETCPAGENICYEMFMVATPK

1462.73 38- 51 GCIDVCPESSLIVK

973.39 52- 59 YVCCNTDR

603.29 33- 37 VPCER

(3) Please help him to identify this toxin. Is it a new toxin?

No. It is a venom produced by "NAJA SPUTATRIX" (MALAYAN SPITTING COBRA).

For more information, please go to http://www.expasy.ch/cgi-bin/get-sprot-entry?Q02454

(4) Please help him to use Prosite scanning tool to find out possible functions or pattern

of this polypeptide.

1 PDOC00005 PS00005 PKC_PHOSPHO_SITE (Protein kinase C phosphorylation site)

Number of matches: 2

1 30-32 TPK

2 57-59 TDR

2 PDOC00245 PS00272 SNAKE_TOXIN (Snake toxins signature)

38-58 GCIDVCPESSLIVKYVCCNTD

(5) David would like to see its structure. Could you help him to find structure of this

toxin or make a model if it is a new protein? Show structure on your homwpage ( 3

different views).