Homework 4
 1. Sho-Ming cloned a gene in the lab. The DNA sequence is listed here: AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAACCCTAAGGTCCTATAGTGTTCTATATCC AACACTAGCTCCTACTCCCTAAAGCATTTATTATATCTTCCCCTAGCTAGATACTTCATTCCACAAATAG TTTGCAGCTTTTTCTTTTCCTCTAAAACAATGGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTG CATGGTGGTAGCTGCACCCTGCGCAGAAGCCATAACCTGTGGCCAGGTTACGTCGAATTTGGCACCTTGT CTTGCTTATCTTAGAAACACGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGTGAATTCTG CAAGGACCACAGAAGATCGTCAAATTGCATGCACTTGCCTGAAATCAGCTGCAGGTGCTATTTCTGGAAT CAATTTGGGCAAAGCTGCTGGTCTCCCTAGTACTTGTGGTGTCAATATTCCTTACAAGATCAGCCCTTCC ACTGACTGCTCCAAGTACCTCACTTTTTTTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTTC ATTTTCGCTTATCTTTTAAATTTTTTATTCGGAATCTTTATACCA Please help him to identify the gene and its protein sequence.
Answer :
   
  1. The gene is the ltp1 gene of Nicotiana tabacum for lipid transferase.
    The whole gene is
  2. It's protein sequence is: 
    MEIAGKIACF VVLCMVVAAP CAEAITCGQV TSNLAPCLAY LRNTGPLGRC CGGVKALVNS
 ARTTEDRQIA CTCLKSAAGA ISGINLGKAA GLPSTCGVNI PYKISPSTDC SKVQ
 2. Please find the gene that encode the trp repressor. Show it mRNA on your home page.
Answer :
    The DNA corrosponds to mRNA is from 329 to 711. The whole sequence is : 
     1 ggatccggaa acgaatatca acattggcac cagttacctg caatatgttt atcagcagtt
      
    61 tggcaataat cgtattttct cctcagcagc ttataacgcc ggactagggc gggtgcgaac
      
   121 ctggcttggc aacagcgccg ggcgtatcga cgcagtggca tttgtcgaga gtattccatt
      
   181 ctccgagacg cgcggttatg tgaagaacgt gctggcttat gacgcttact accgctattt
     
   241 catgggggat aaaccgacgt tgatgagcgc cacggaatgg ggacgtcgtt actgatccgc
    
   301 acgtttatga tatgctatcg tactctttag cgagtacaac cgggggaggc attttgcttc
      
   361 ccccgctaac aatggcgaca tattatggcc caacaatcac cctattcagc agcgatggca
      
   421 gaacagcgtc accaggagtg gttacgtttt gtcgacctgc ttaagaatgc ctaccaaaac
      
   481 gatctccatt taccgttgtt aaacctgatg ctgacgccag atgagcgcga agcgttgggg
      
   541 actcgcgtgc gtattgtcga agagctgttg cgcggcgaaa tgagccagcg tgagttaaaa
      
   601 aatgaactcg gcgcaggcat cgcgacgatt acgcgtggat ctaacagcct gaaagccgcg
      
   661 cccgtcgagc tgcgccagtg gctggaagag gtgttgctga aaagcgattg attttgtagg
      
   721 cctgataaga cgtggcgcat caggcatcgt gcaccgaatg ccggatgcgg cgtgaaggcc
      
   781 ttatccgtcc tacaaatacc cgtaatttca atatgtttgg taggcatgat aagacgcggc
      
   841 agcgtcgcat caggcgctta atacacggca ttatgaaacg gactcagcgc caggatcacc
      
   901 gcctggtgat agacgctggc gcgagtgagt ttcccggcgg taaacacgcc gatcgcccct
   
   961 tccttacgac cgatctcatc aataccggta taacgcgaca tcacgggacc aagcgcctca
     
  1021 ccttcacgca ctttttccag aatcaccgca ggcaacggca aagtagccga acgcgcctcg
     
  1081 ccgcgctggc tggcgttttc aatcaccacc caactgaaag tgctgtcacc atcgatgcca
     
  1141 gcttcaatcg ccacccaaaa atcagcctct ggaagtaaac ggcgggcatt ggctacccga
     
  1201 tttcgtgcgc cagcgcgcgt ttcctcactg ccaaagggct gttccggtac accgctctcg
     
  1261 acggcaacgg atgcaatatg gcaggatcc

 3. How many nucleotide and protein sequence of Lycopersicon esculentum were know? Please find its class II small heat shock protein mRNA, complete cds.
Answer :
   
  1. The number of nucleotide know is : 679.
    The number of protein know is : 832.
  2. The DNA corrosponds to mRNA is from 1 to 738.
       1 tacggctgcg agaagacgac agaaggggac tgcaattaca aatcaaacca aaattgacaa
      
  61 atttcacgca caaaatctca atgtccaaaa atttctcaat actgaaaatg gatttgaggt
      
 121 tgttgggtat cgataacaca ccactcttcc acactctcca ccatatgatg gaagctgccg
      
 181 gtgaagattc cgacaagtct gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg
      
 241 ccatggctgc tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg
      
 301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac aatgtgctgt
      
 361 tgattagtgg tgaaaggaag aggggagaag agaaagaagg tgcaaagttt attaggatgg
      
 421 agagaagggt tgggaaattc atgaggaagt ttagtctgcc agagaatgcg aatactgatg
      
 481 caatttctgc agtttgtcaa gatggagttc tgactgttac tgttcagaaa ttgcctcctc
     
 541 ctgagccaaa gaaacccaaa acaattgagg tgaaagttgc ttgaagttat ggactctatt
      
 601 ttgatggttt gtggtatgat gtagtagaaa taaagttgta ggagtagtga acttttcctt
      
 661 tcatctttct gctatgtttt cacgtctgtt tgaatgttac aatagccatg ggtattgttt
      
 721 gttttgatgc caaaaaaa
    The whole DNA of the classII small heat shock protein is