Homework 6 
David Liu, same guy from last homework, he also isolated a cDNA clone from snake venom library. The nucleotide
sequence of this cDNA clone is shown here:

AAAACCATCAAATACGTTATGCTGGAATGCAACGAACTGATCCCGCTGTTCTACGAAACCT

GCCCGGCTGGTGAAAACATCTGCTACGAAATGTTCATGGTTGCTACCCCGAAAGTTCCGTGC

GAACGTGGTTGCATCGACGTTTGCCCGGAATCTTCTCTGATCGTTAAATACGTTTGCTGCAA

CACCGACCGTTGCCAGTAATCCAGCGCCTGATCTCTCGAAATAAAAGCCGCATTG 

(1) Please help him to analyze the cutting patterns of following restriction enzyme: 

     EcoRI, Sau3AI, HinfI and MseI.

Answer :
The cutting numbers : EcoRI 0, Sau3AI 3, HinfI 1, and MseI 1.
More complete information

(2) Please help him to find its corresponding polypeptide sequence.
Answer :
DNA Sequence
AAAACCATCAAATACGTTATGCTGGA ATGCAACGAACTGATCCCGCTGTTCTACGAAACCT GCCCGGCTGGTGAAAACATCTGCTACGAAATGTTCATGGTTGCTACCCCGAAAGTTCCGTGC GAACGTGGTTGCATCGACGTTTGCCCGGAATCTTCTCTGATCGTTAAATACGTTTGCTGCAA CACCGACCGTTGCCAGTAATCCAGCGCCTGATCTCTCGAAATAAAAGCCGCATTG

Polypeptide Sequence
 MLECNELIPL FYETCPAGEN ICYEMFMVAT PKVPCERGCI DVCPESSLIV KYVCCNTDRC Q*

(3) Please help him to identify this toxin. Is it a new toxin?
Answer : Yes, it is a new toxin.
NCBI Blast Result

(4) David would like to see its structure. Could you help him to find structure of this toxin or make a model if it is a new protein? Show structure on your homwpage ( 3 different views).
red : Helice
yellow : sheets
cyan : turns