Query Information

ORIGIN animal
BEGIN
>TEST
 LKCNKLVPLF YKTCPAGKNL CYKMFMVATP KVPVKRGCID VCPKSSLLVK
 YVCCNTDRCN 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
TEST          60 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 4

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   6
     Peak Value of UR:   0.89
     Net Charge of CR: 2
     Discriminant Score:     -8.83
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.67
     Possible cleavage site: 37
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.24  threshold: -2.0
     PERIPHERAL  Likelihood =  4.24
     modified ALOM score:  -1.75
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 38
     KRGCID
Discrimination of mitochondrial target seq.: 
     negative (-2.94)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
SKL motif (signal for peroxisomal protein): 
     pos: -1(60), count: 0   
Amino Acid Composition Tendency for Peroxisome: -12.44
Peroxisomal proteins?   Status: negative
Amino Acid Composition tendency for lysosomal proteins
     score: -4.00  Status: negative
Modified score for lysosome: 0.100
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score1: 0.650

----- Final Results -----

                        cytoplasm --- Certainty= 0.650(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
                 lysosome (lumen) --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----