1. Sho-Ming cloned a gene in the lab. The DNA sequence is

listed here:

AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAACCCTAAGGTCCTATAGTGTTCTATATCCAACACTAGCTCCTACTCCCTAAAGCATTTATTATATCTTCCCCTAGCTAGATACTTCATTCCACAAATAGTTTGCAGCTTTTTCTTTTCCTCTAAAACAATGGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTGCATGGTGGTAGCTGCACCCTGCGCAGAAGCCATAACCTGTGGCCAGGTTACGTCGAATTTGGCACCTTGTCTTGCTTATCTTAGAAACACGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGTGAATTCTGCAAGGACCACAGAAGATCGTCAAATTGCATGCACTTGCCTGAAATCAGCTGCAGGTGCTATTTCTGGAATCAATTTGGGCAAAGCTGCTGGTCTCCCTAGTACTTGTGGTGTCAATATTCCTTACAAGATCAGCCCTTCCACTGACTGCTCCAAGTACCTCACTTTTTTTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTTCATTTTCGCTTATCTTTTAAATTTTTTATTCGGAATCTTTATACCA

Please help him to identify the gene and its protein

sequence.

ANS:

DEFINITION lipid transferase.

ACCESSION 19883

PID g19883

DBSOURCE EMBL: locus NTLTP1, accession X62395

SOURCE common tobacco.

ORGANISM Nicotiana tabacum

gi|19883 lipid transferase

MEIAGKIACFVVLCMVVAAPCAEAITCGQVTSNLAPCLAYLRNTGPLGRCCGGVKALVNSARTTEDRQIATCLKSAAGAISGINLGKAAGLPSTCGVNIPYKISPSTDCSKVQ

2. How many nucleotide and protein sequence of Lycopersicon esculentum were know?

Please find its class II small heat shock protein mRNA, complete cds.

ANS:

Nucleotide (742) Protein (1019)

1 tacggctgcg agaagacgac agaaggggac tgcaattaca aatcaaacca

51 aaattgacaa atttcacgca caaaatcaca atatccaaaa atttctcaat

101 actgaaaatg gatttgaggt tgttgggtat cgataacaca ccactcttcc

151 acactctcca ccatatgatg gaagctgccg gtgaagattc cgacaagtct

201 gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg ccatggctgc

251 tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg

301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac

351 aatgtgctgt tgattagtgg tgaaaggaag agggaagaag agaaagaagg

401 tgcaaagttt attaggatgg agagaagggt tgggaaattc atgaggaagt

451 ttagtctgcc agagaatgcg aatactgatg caatttctgc agtttgtcaa