1. Sho-Ming cloned a gene in the lab. The DNA sequence is listed here:
Please help him to identify the gene and its protein sequence.
AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAACCCTAAGGTCCTATAGTGTTCTATATCC
AACACTAGCTCCTACTCCCTAAAGCATTTATTATATCTTCCCCTAGCTAGATACTTCATTCCACAAATAG
TTTGCAGCTTTTTCTTTTCCTCTAAAACAATGGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTG
CATGGTGGTAGCTGCACCCTGCGCAGAAGCCATAACCTGTGGCCAGGTTACGTCGAATTTGGCACCTTGT
CTTGCTTATCTTAGAAACACGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGTGAATTCTG
CAAGGACCACAGAAGATCGTCAAATTGCATGCACTTGCCTGAAATCAGCTGCAGGTGCTATTTCTGGAAT
CAATTTGGGCAAAGCTGCTGGTCTCCCTAGTACTTGTGGTGTCAATATTCCTTACAAGATCAGCCCTTCC
ACTGACTGCTCCAAGTACCTCACTTTTTTTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTTC
ATTTTCGCTTATCTTTTAAATTTTTTATTCGGAATCTTTATACCA
Solution:
Gene:
N.tabacum ltp1 gene for lipid transferase
Plus Strand HSPs:
Score = 2976 (822.3 bits), Expect = 2.1e-243, Sum P(2) = 2.1e-243
Identities = 598/605 (98%), Positives = 598/605 (98%), Strand = Plus / Plus
Protein Sequence:
MEIAGKIACF VVLCMVVAAP CAEAITCGQV TSNLAPCLAY LRNTGPLGRC CGGVKALVNS ARTTEDRQIA CTCLKSAAGA ISGINLGKAA GLPSTCGVNI PYKISPSTDC SKVQ
2. Please find the gene that encode the trp repressor. Show its mRNA on your home page.
Solution:
E.coli trpR gene coding for the trp operon repressor protein.
mRNA 329..711
¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@329 ag cgaguacaac cgggggaggc auuuugcuuc
361 ccccgcuaac aauggcgaca uauuauggcc caacaaucac ccuauucagc agcgauggca
421 gaacagcguc accaggagug guuacguuuu gucgaccugc uuaagaaugc cuaccaaaac
481 gaucuccauu uaccguuguu aaaccugaug cugacgccag augagcgcga agcguugggg
541 acucgcgugc guauugucga agagcuguug cgcggcgaaa ugagccagcg ugaguuaaaa
601 aaugaacucg gcgcaggcau cgcgacgauu acgcguggau cuaacagccu gaaagccgcg
661 cccgucgagc ugcgccagug gcuggaagag guguugcuga aaagcgauug a 711
3. How many nucleotide and protein sequence of Lycopersicon esculentum were know? Please find its class II small heat shock protein mRNA, complete cds.
Solution:
Nucleotide (679) Protein (832)
Lycopersicon esculentum class II small heat shock protein Le-HSP18.6 mRNA, complete cds.
1 tacggctgcg agaagacgac agaaggggac tgcaattaca aatcaaacca aaattgacaa
61 atttcacgca caaaatctca atgtccaaaa atttctcaat actgaaaatg gatttgaggt
121 tgttgggtat cgataacaca ccactcttcc acactctcca ccatatgatg gaagctgccg
181 gtgaagattc cgacaagtct gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg
241 ccatggctgc tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg
301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac aatgtgctgt
361 tgattagtgg tgaaaggaag aggggagaag agaaagaagg tgcaaagttt attaggatgg
421 agagaagggt tgggaaattc atgaggaagt ttagtctgcc agagaatgcg aatactgatg
481 caatttctgc agtttgtcaa gatggagttc tgactgttac tgttcagaaa ttgcctcctc
541 ctgagccaaa gaaacccaaa acaattgagg tgaaagttgc ttgaagttat ggactctatt
601 ttgatggttt gtggtatgat gtagtagaaa taaagttgta ggagtagtga acttttcctt
661 tcatctttct gctatgtttt cacgtctgtt tgaatgttac aatagccatg ggtattgttt
721 gttttgatgc caaaaaaa