Grail Overview

GRAIL VERSIONS


GRAIL 1 
1. It recognizes coding potentialwithin a fixed size (100 base) window. 

2. It evaluates coding potential without looking for additional features (information such as splice junctions, etc). 


GRAIL 1a (an updated version of GRAIL 1) 
It evaluates a number of discrete candidates of different lengths around each potential 
coding region, usinginformation from the two 60-base regions adjacent to that coding 
region.


GRAIL 2  
1. It uses variable-length windows tailored to each potential exon 
candidate(ORF)bounded by a pair of start/donor, acceptor/donor or acceptor/stop sites. 

2. GRAIL 2 is not appropriate for sequences without genomic context 




Miscellaneous

1. Initial Coding Scores: 
   
   Scores range : 0.0 - 1.0 .( > 0.5 identifies a region with protein encoding potential.) 
   
   Non-coding regions : 0.000.

2. Models of genes can be constructed only from GRAIL 2 exons. 

3. Database searches and protein translations can be done from any version.