3. It's a new toxin but shares some similarities with known protein cardiotoxin.
4. The structure proposed by known bases are show below: (helix=yellow, sheet=blue)
Cartoon View
Sticks View
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1. 1) EcoRI doesn't have a cutting site on this sequence.
2) Sau3AI 'GATC_ - 3 Cut(s) : 39 162 215
3) HinfI G'AnT_C - 1 Cut(s): 152
4) MseI T'TA_A - 1 Cut(s): 168
The translated protein sequences are also provided here.
AlwI AciI
CviRISau3AIMspA1I Pfl1108I
BccI MaeII BsmI DpnI FauI
\ \ \ \ \ \ \ \ \ \ \
1 aaaaccatcaaatacgttatgctggaatgcaacgaactgatcccgctgttctacgaaacc 60
ttttggtagtttatgcaatacgaccttacgttgcttgactagggcgacaagatgctttgg
^ * ^ * ^ * ^ * ^ * ^ *
K T I K Y V M L E C N E L I P L F Y E T
CviJI
MspI
ScrFI
NciI Pfl1108I
BspMI HphI NlaIII
\ \ \ \ \
61 tgcccggctggtgaaaacatctgctacgaaatgttcatggttgctaccccgaaagttccg 120
acgggccgaccacttttgtagacgatgctttacaagtaccaacgatggggctttcaaggc
^ * ^ * ^ * ^ * ^ * ^ *
C P A G E N I C Y E M F M V A T P K V P
MboII TfiI
MspI
MaeII ScrFI MseI
TaqI NciI Sau3AIBbvI TseI
MaeII CviRI SfaHinfIDpnI MaeII Fnu4HI
\ \ \ \ \ \ \ \ \ \ \ \ \ \\
121 tgcgaacgtggttgcatcgacgtttgcccggaatcttctctgatcgttaaatacgtttgc 180
acgcttgcaccaacgtagctgcaaacgggccttagaagagactagcaatttatgcaaacg
^ * ^ * ^ * ^ * ^ * ^ *
C E R G C I D V C P E S S L I V K Y V C
Sau3AI AciI
BsrI HhaI DpnI TauI
BsbI Tsp4CI HaeII Fnu4HI
CviRI BsiEI AlwNI TaqI CviJI
\ \ \\ \ \\ \ \ \ \\
181 tgcaacaccgaccgttgccagtaatccagcgcctgatctctcgaaataaaagccgcatt 240
acgttgtggctggcaacggtcattaggtcgcggactagagagctttattttcggcgtaa
^ * ^ * ^ * ^ * ^ * ^ *
C N T D R C Q Z S S A Z S L E I K A A
2. Translated polypeptide sequence are as follows:
K T I K Y V M L E C N E L I P L F Y E T C P A G E N I C Y E M F
M V A T P K V P C E R G C I D V C P E S S L I V K Y V C C N T D
R C Q Z S S A Z S L E I K A A
3. It's a new toxin but shares some similarities with known protein cardiotoxin.
4. The structure proposed by known bases are show below: (helix=yellow, sheet=blue)
Cartoon View
Sticks View
Wireframe View