在NCSA Biology Workbench 中有兩種方法可以預測secondary protein structures.

CHOFAS Predict secondary protein structure (Chou-Fasman plot)

 60 aa;                 
             .         .         .         .         .         .
       LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
 helix <--------->        <------------->       <-------->         
 sheet     EEEEEEEEE      EEEEEEEEEE        EEEE     EEEEEEEEE     
 turns                  T           T     T       TT               
              


 Residue totals: H: 36   E: 32   T:  5
        percent: H: 60.0 E: 53.3 T:  8.3

GARNIER Results
Predict secondary structure of protein sequences using the method
of Garnier, Osgusthorpe, and Robinson, J. Mol. Biol.

Garnier plot of cytotoxin 3 - Chinese cobra

 60 aa; DCH = 0, DCS = 0
 
           .   10    .   20    .   30    .   40    .   50    .   60
       LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
 helix H                 HHH     HHHH                              
 sheet     EEEEEEEEE        EEEEE      E E  EEEEE    EEEEEEEE      
 turns  TTT          TTTT            T  T TT     TTTT        TTTTTT
 coil               C                 C                            
       
  

 Residue totals: H:  8   E: 29   T: 21   C:  2
        percent: H: 18.2 E: 65.9 T: 47.7 C:  4.5
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