1  0+00+00000 0+00000+00 00+0000000 +000++000- 000+00000+ 000000-+00

A. CHARGE CLUSTERS.

 Positive charge clusters (cmin = 13/30 or 18/45 or 22/60):  none
 

Negative charge clusters:  not evaluated (frequency of - < 5%, too low)
 

Mixed charge clusters (cmin = 15/30 or 20/45 or 25/60):  none
 

B. HIGH SCORING (UN)CHARGED SEGMENTS.
 
 
 

High scoring positive charge segments:

score=   2.00 frequency=   0.183  ( KR )
score=   0.00 frequency=   0.000  ( BZX )
score=  -1.00 frequency=   0.783  ( LAGSVTIPNFQYHMCW )
score=  -2.00 frequency=   0.033  ( ED )

 Expected score/letter:  -0.483
 - now scoring for positive charge segments;    Average information/letter:   0.430
 Minimal length of displayed segments set to:  20

M_0.01= 13.07  (cv=  7.77, lambda=  0.52686, k=  0.16371, x=  5.30;
                90% confidence interval for segment length:  23 +-  25)
M_0.05=  9.97  (x=  2.20)

# of segments (>=20 residues) exceeding M_0.05: none
 
 
 

High scoring negative charge segments:

score=   2.00 frequency=   0.033  ( ED )
score=   0.00 frequency=   0.000  ( BZX )
score=  -1.00 frequency=   0.783  ( LAGSVTIPNFQYHMCW )
score=  -2.00 frequency=   0.183  ( KR )

 Expected score/letter:  -1.083
 - now scoring for negative charge segments;    Average information/letter:   3.490
 Minimal length of displayed segments set to:  20

M_0.01=  4.94  (cv=  2.54, lambda=  1.61122, k=  0.47671, x=  2.40;
                90% confidence interval for segment length:   3 +-   3)
M_0.05=  3.92  (x=  1.38)

# of segments (>=20 residues) exceeding M_0.05: none
 
 
 

High scoring mixed charge segments:

score=   1.00 frequency=   0.217  ( KEDR )
score=   0.00 frequency=   0.000  ( BZX )
score=  -1.00 frequency=   0.783  ( LAGSVTIPNFQYHMCW )

 Expected score/letter:  -0.567
 - now scoring for mixed charge segments;    Average information/letter:   1.051
 Minimal length of displayed segments set to:  20

M_0.01=  6.07  (cv=  3.19, lambda=  1.28520, k=  0.40993, x=  2.89;
                90% confidence interval for segment length:  11 +-   9)
M_0.05=  4.80  (x=  1.62)

# of segments (>=20 residues) exceeding M_0.05: none
 
 
 

High scoring uncharged segments:

score=   1.00 frequency=   0.783  ( LAGSVTIPNFQYHMCW )
score=   0.00 frequency=   0.000  ( BZX )
score=  -8.00 frequency=   0.217  ( KEDR )

 Expected score/letter:  -0.950
 - now scoring for uncharged segments;    Average information/letter:   0.173
 Minimal length of displayed segments set to:  20

M_0.01= 32.53  (cv= 20.56, lambda=  0.19916, k=  0.10900, x= 11.97;
                90% confidence interval for segment length:  54 +-  44)
M_0.05= 24.34  (x=  3.79)

# of segments (>=20 residues) exceeding M_0.05: none
 

C. CHARGE RUNS AND PATTERNS.

pattern  (+)|  (-)|  (*)|  (0)| (+0)| (-0)| (*0)|(+00)|(-00)|(*00)|
lmin0     5 |   3 |   6 |  29 |  10 |   6 |  10 |  11 |   6 |  12 |
lmin1     6 |   4 |   7 |  36 |  12 |   7 |  12 |  14 |   8 |  14 |
lmin2     7 |   4 |   8 |  39 |  13 |   8 |  14 |  15 |   9 |  16 |

There are no charge runs or patterns exceeding the given minimal lengths.

Run count statistics:

  +  runs >=   3:   0
  -  runs >=   3:   0
  *  runs >=   4:   0
  0  runs >=  20:   0