ProtParam tool

Number of amino acids: 390

Molecular weight: 43417.8

Theoretical pI: 5.35

Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 58


Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

                                         -1   -1
Extinction coefficients are in units of M   cm .

The first table lists values computed assuming ALL Cys 
residues appear as half cysteines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    23885   24181   24065   23830   23160
Abs 0.1% (=1 g/l)   0.550   0.557   0.554   0.549   0.533



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    23450   23800   23705   23470   22800
Abs 0.1% (=1 g/l)   0.540   0.548   0.546   0.541   0.525


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hour (mammalian reticulocytes, in vitro).
                            >20 hour (yeast, in vivo).
                            >10 hour (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 60.61
This classifies the protein as unstable.



Aliphatic index: 62.92

Grand average of hydropathicity (GRAVY): -0.840