Denaturation

Equilibrium Denaturation Experiment

Figure adopted from Grantcharova and Baker, Biochemistry, 1997, 36(50):15685
	Equilibrium GdmCl denaturation followed by fluorescence (filled circle) and CD (filled triangle) at 295 K. Fluorescence was recorded at 225 nm and CD at 235 nm. Solid lines are the fits to the experimental data using the linear extrapolation model. Baselines were corrected for sloping before conversion to fraction folded.
	signal = N₀ + C_ntT + C_ngD +[(D₀+D_ntT+D_ngD)exp(-G/RT)]/[1+exp(-G/RT)] (1) where G_U = H_g + mD - TS_g C_p [T_g - T + T ln(T/T_g)] (2)
	Figure adopted from Grantcharova and Baker, Biochemistry, 1997, 36(50):15685
Three-dimensional guanidine-temperature denaturation surface. Closed circles represent experimentally determined values from the individual temperature melts at different GdmCl concentrations. Continuous net is the best fit of eq 1 or 2 to the experimental data. The G_U estimates from two models were similar (4.7 +/- 0.22 and 4.44 +/- 0.2 kcal/mol). [Note] The 3D denaturation method reduces thhe uncertainties in estimations quanties in 0 M denaturant since the extroplations are based on more data compared to a GdmCl denaturation experiment at a single temperature.