homework 5 answer 1

1. Please find the 1.9 Å crystal structure of the Aequorea victoria green fluorescent protein (GFP) from PDB. Give the following informations:
- a.) PDB ID number.
  Ans: 1EMA.
- b.) Name of the authors.
  Ans: M.Ormo, A.B.Cubitt, K.Kallio, L.A.Gross, R.Y.Tsien, S.J.Remington.
- c.) Expression system used by authors.
```
Ans: Expression_system: Escherichia Coli
        Expression_system_strain: Jm109 (De3)
```
- d.) Residue number.
  Ans: 236 residues.
- e.) Number of helix.
  Ans: 4 helices.
- f.) The starting residue and ending residue of each helix.
```
Ans:            ( start ,  end  )
        helix 1:  GLY4 ,  PHE8
        helix 2: TRP57, LEU60
        helix 3: GLN69, PHE71
        helix 4: PHE83, SER86
```
- g.) Number of b-sheet.
  Ans: 11 b-sheet.