2. The same technique can be used to assay cellulase enzyme activity e.g. Novo Nordisk
    Celluclast.

3. The course of cellulase breakdown can be followed over several days by using
    duplicate plates.
 
 

 
Cellulomonas sp. growing on filter paper (electron micrograph taken after 3 days of
incubation).
 

 
 

 
 

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                                                                                                                              1. Sterilize a cork borer by flaming it with alcohol
 
 
 

2. Cut and remove a plug of agar from a plate, forming a
                                               well
 
 
 

3. Make another well in the same way Inoculate one well
                                           with culture and the other with water
 
 
 

      4. Incubate the plate the right way up
 
 
 

        5. Stain and observe the plate after 2 days Measure diameter
                                          of zone in which cellulose has been broken down