The slope culture should be used to prepare several nutrient broth cultures in McCartney bottles for classroom use. This should be done two or three days before it is intended to carry out this practical work, and the bottles should be incubated at 25-30oC.
Sterile Petri dishes
For each Petri dish, 15
ml of CMC agar,
made with 0.5 g
carboxymethylcellulose
(a soluble form
of cellulose); 0.1 g
NaNO3
; 0.1 g K
2 HPO4 ; 0.1
g KCl;
0.05 g MgSO4
; 0.05 g yeast
extract; and
0.1 g glucose
in 100 ml of
water. The medium
should be solidified using 1.7 %
w/v agar.
Sterile water (dispensed into a McCartney bottle)
Sterile 1 ml syringes (without needles), 2
Discard beaker containing 5% solution of Chlorate I solution
Congo red solution (made with 1 mg per ml of water)
1 M sodium chloride solution
Ethanol (for flaming cork borer)
Cork borer, 5 mm diameter
Bunsen burner
Incubator set at 25-30 oC
Pen
for marking Petri dishes
