Materials  
         
      Round green lettuce, 1 leaf

      21% sucrose solution, 0.1 cm3

      13% sorbitol solution, 20 cm3

      Carbohydrase enzyme mixture, Novo Viscozyme , 0.5 cm3

      Paper tissue

      10 cm3 plastic syringe

      1 cm3 plastic syringes, 2

      Test tube

      Centrifuge tube

      Fine glass stirring rod

      Filter funnel

      Strip of nylon gauze, to plug filter funnel

      Microscope slide

      Cover slip

      Microscope with x40 objective

      Water bath set at 37oC

      Bench centrifuge