Ans:
在NCBI的 blast中查到此段sequence為tobacco(煙草)中的一段gene。
請看完整的查詢結果(the whole imformation)
andthe whole gene sequence。
其translate之後之 protein為lipid transferase(完整的資料)。
其sequence為:MEIAGKIACFVVLCMVVAAPCAEAITCGQVTSNLAPCLAYLRNT
GPLGRCCGGVKALVNSARTTEDRQIACTCLKSAAGAISGINLGKA
AGLPSTCGVNIPY
KISPSTDCSKVQ
題目2. How many nucleotide and protein sequence of Lycopersicon
esculentum were know?
Please find its class II small heat shock protein mRNA,
complete cds.
Ans:在NCBI的 Taxonomy 中查到:Lycopersicon esculentum即 tomato(番茄)
know sequence:nucleotide共:817個,protein共 1092個。(until 1997/12/8)
請看完整的
查詢結果.
接著在上述之查詢結果同一頁下方可以Entrez查所有的proteins,再以key word"heat shock protein"限制查詢,得24個proteins,顯示出來之後可以找到所要的
class II small heat shock protein
而在其DBSOURCE中U72396可link至
此protein之mRNA
其完整的cds如下:
CDS 108..584
/note="heat treatment/chilling tolerance related protein
from tomato fruit"
/codon_start=1
/product="class II small heat shock protein Le-HSP17.6"
/db_xref="PID:g1773291"
/translation="MDLRLLGIDNTPLFHTLHHMMEAAGEDSDKSVNAPSRNYVRDAK
AMAATPADVKEYPNSYVFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKFI
RMERRVGKFMRKFSLPENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKVA"