Mol Cell Biol 5 (4): 831-838 (Apr 1985)

Isolation of duplicated human c-src genes located on

chromosomes 1 and 20.

Parker RC, Mardon G, Lebo RV, Varmus HE, Bishop JM

The oncogene (v-src) of Rous sarcoma virus apparently arose by transduction of the chicken

gene known as c-src(chicken). We isolated DNA fragments representative of two src-related loci

from recombinant DNA bacteriophage libraries of the human genome. One of these loci,

c-src1(human), appeared to direct the synthesis of a 5-kilobase polyadenylated RNA that

presumably encodes pp60c-src(human). Probes specific for the other locus, c-src2(human), did

not hybridize to polyadenylated RNA prepared from a variety of human cell lines. Partial

nucleotide sequence determinations of the loci demonstrated that c-src1(human) is highly related

to chicken c-src and that c-src2(human) is slightly more divergent. The sequences imply that the

final two coding exons of each human locus are identical in length to those of chicken c-src and

that the location of an amber stop codon is unchanged in all three loci. c-src1(human) has been

mapped to chromosome 20, and the second locus is located on chromosome 1. We conclude that

c-src1(human) is the analog of c-src(chicken) and that the duplicated locus, c-src2(human), may

also be expressed.

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