Homework 4
(1) Use NCBI BLASTN to search, and the result:
gb:NTLTP1 N.tabacum ltp1 gene for lipid transferase.
Length = 2075
Plus Strand HSPs:
Score = 3025 (835.9 bits), Expect = 7.9e-248,
Sum P(2) = 7.9e-248
Identities = 605/605 (100%), Positives = 605/605 (100%),
Strand = Plus / Plus
translation="MEIAGKIACFVVLCMVVAAPCAEAITCGQVTSNLAPCLAYLRNT
GPLGRCCGGVKALVNSARTTEDRQIACTCLKSAAGAISGINLGK
AAGLPSTCGVNIPYKISPSTDCSKVQ"
(2) (mRNA for Trp repressor is shown in blue)
LOCUS ECOTRPR 1289 bp DNA BCT 22-SEP-1986
DEFINITION E.coli trpR gene coding for the trp operon repressor protein.
ACCESSION J01715 V00369
NID g148059
KEYWORDS DNA-binding protein; repressor; trp repressor; trpR gene.
SOURCE Escherichia coli DNA [1],[2] and mRNA [2].
ORGANISM Escherichia coli
Eubacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
Escherichia.
REFERENCE 1 (bases 1 to 1041)
AUTHORS Singleton,C.K., Roeder,W.D., Bogosian,G., Somerville,R.L. and
Weith,H.L.
JOURNAL Nucleic Acids Res. 8, 1551-1560 (1980)
1 ggatccggaa acgaatatca acattggcac cagttacctg caatatgttt atcagcagtt
61 tggcaataat cgtattttct cctcagcagc ttataacgcc ggactagggc gggtgcgaac
121 ctggcttggc aacagcgccg ggcgtatcga cgcagtggca tttgtcgaga gtattccatt
181 ctccgagacg cgcggttatg tgaagaacgt gctggcttat gacgcttact accgctattt
241 catgggggat aaaccgacgt tgatgagcgc cacggaatgg ggacgtcgtt actgatccgc
301 acgtttatga tatgctatcg tactctttag cgagtacaac cgggggaggc attttgcttc
361 ccccgctaac aatggcgaca tattatggcc caacaatcac cctattcagc agcgatggca
421 gaacagcgtc accaggagtg gttacgtttt gtcgacctgc ttaagaatgc ctaccaaaac
481 gatctccatt taccgttgtt aaacctgatg ctgacgccag atgagcgcga agcgttgggg
541 actcgcgtgc gtattgtcga agagctgttg cgcggcgaaa tgagccagcg tgagttaaaa
601 aatgaactcg gcgcaggcat cgcgacgatt acgcgtggat ctaacagcct gaaagccgcg
661 cccgtcgagc tgcgccagtg gctggaagag gtgttgctga aaagcgattg attttgtagg
721 cctgataaga cgtggcgcat caggcatcgt gcaccgaatg ccggatgcgg cgtgaaggcc
781 ttatccgtcc tacaaatacc cgtaatttca atatgtttgg taggcatgat aagacgcggc
841 agcgtcgcat caggcgctta atacacggca ttatgaaacg gactcagcgc caggatcacc
901 gcctggtgat agacgctggc gcgagtgagt ttcccggcgg taaacacgcc gatcgcccct
961 tccttacgac cgatctcatc aataccggta taacgcgaca tcacgggacc aagcgcctca
1021 ccttcacgca ctttttccag aatcaccgca ggcaacggca aagtagccga acgcgcctcg
1081 ccgcgctggc tggcgttttc aatcaccacc caactgaaag tgctgtcacc atcgatgcca
1141 gcttcaatcg ccacccaaaa atcagcctct ggaagtaaac ggcgggcatt ggctacccga
1201 tttcgtgcgc cagcgcgcgt ttcctcactg ccaaagggct gttccggtac accgctctcg
1261 acggcaacgg atgcaatatg gcaggatcc
mRNA : 329 ~ 711
(3)
Nucleotide (738)
Protein (167)
LOCUS LEU72396 738 bp mRNA PLN 23-OCT-1996
DEFINITION Lycopersicon esculentum class II small heat shock protein
Le-HSP18.6 mRNA, complete cds.
ACCESSION U72396
NID g1622944
KEYWORDS .
SOURCE tomato.
ORGANISM Lycopersicon esculentum
Eukaryotae; mitochondrial eukaryotes; Viridiplantae;
Charophyta/Embryophyta group; Embryophyta; Magnoliophyta;
Magnoliopsida; Solananae; Solanales; Solanaceae; Solanum clade;
Lycopersicon.
REFERENCE 1 (bases 1 to 738)
AUTHORS Kadyrzhanova,D.K., Vlachonasios,K.E., Ververidis,P. and Dilley,D.R.
TITLE A heat-treatment chilling tolerance related cDNA from tomato fruit
encoding a small heat-shock protein class II
JOURNAL Unpublished (1996)
REFERENCE 2 (bases 1 to 738)
AUTHORS Kadyrzhanova,D.K., Vlachonasios,K.E., Ververidis,P. and Dilley,D.R.
TITLE Direct Submission
JOURNAL Submitted (24-SEP-1996) Horticulture, Michigan State University,
Plant and Soil Science Building, East Lansing, MI 48824-1325, USA
FEATURES Location/Qualifiers
polyA_signal 629..634
BASE COUNT 227 a 117 c 184 g 210 t
ORIGIN
1 tacggctgcg agaagacgac agaaggggac tgcaattaca aatcaaacca aaattgacaa
61 atttcacgca caaaatctca atgtccaaaa atttctcaat actgaaaatg gatttgaggt
121 tgttgggtat cgataacaca ccactcttcc acactctcca ccatatgatg gaagctgccg
181 gtgaagattc cgacaagtct gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg
241 ccatggctgc tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg
301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac aatgtgctgt
361 tgattagtgg tgaaaggaag aggggagaag agaaagaagg tgcaaagttt attaggatgg
421 agagaagggt tgggaaattc atgaggaagt ttagtctgcc agagaatgcg aatactgatg
481 caatttctgc agtttgtcaa gatggagttc tgactgttac tgttcagaaa ttgcctcctc
541 ctgagccaaa gaaacccaaa acaattgagg tgaaagttgc ttgaagttat ggactctatt
601 ttgatggttt gtggtatgat gtagtagaaa taaagttgta ggagtagtga acttttcctt
661 tcatctttct gctatgtttt cacgtctgtt tgaatgttac aatagccatg ggtattgttt
721 gttttgatgc caaaaaaa
LOCUS 1622945 167 aa 21-OCT-1996
DEFINITION class II small heat shock protein Le-HSP18.6.
ACCESSION 1622945
PID g1622945
DBSOURCE GENBANK: locus LEU72396, accession U72396
KEYWORDS .
SOURCE tomato.
ORGANISM Lycopersicon esculentum
Eukaryotae; mitochondrial eukaryotes; Viridiplantae;
Charophyta/Embryophyta group; Embryophyta; Magnoliophyta;
Magnoliopsida; Solananae; Solanales; Solanaceae; Solanum clade;
Lycopersicon.
REFERENCE 1 (residues 1 to 167)
AUTHORS Kadyrzhanova,D.K., Vlachonasios,K.E., Ververidis,P. and Dilley,D.R.
TITLE A heat-treatment chilling tolerance related cDNA from tomato fruit
encoding a small heat-shock protein class II
JOURNAL Unpublished (1996)
REFERENCE 2 (residues 1 to 167)
AUTHORS Kadyrzhanova,D.K., Vlachonasios,K.E., Ververidis,P. and Dilley,D.R.
TITLE Direct Submission
JOURNAL Submitted (24-SEP-1996) Horticulture, Michigan State University,
Plant and Soil Science Building, East Lansing, MI 48824-1325, USA
FEATURES Location/Qualifiers
ORIGIN
1 msknfsilkm dlrllgidnt plfhtlhhmm eaagedsdks vnapsrnyvr dakamaatpa
61 dvkeypnsyv fvvdmpglks gdikvqveed nvllisgerk rgeekegakf irmerrvgkf
121 mrkfslpena ntdaisavcq dgvltvtvqk lpppepkkpk tievkva
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