Homework 5

(1)此毒素是一種可在Cardiotoxin iii 中發現的蛋白質
   有兩種完全符合的結構：   2crs (NMR 13 structures)

   2crt (minimized average structure)




(2) 含有此sequence之蛋白質如下：

Cardiotoxin iii (NMR minimized average structure)
Cardiotoxin iii (NMR 13 structures)
Cardiotoxin ii (NMR 12 structures)
Cardiotoxin ii (NMR minimized average structure)
Cardiotoxin ctx i (NMR 11 structures)
Cardiotoxin v=4===II== (toxin III)
Cardiotoxin gamma (NMR minimized average structure)
Toxin gamma (cardiotoxin)
Cardiotoxin v=4===II== (toxin III)
Toxin gamma (cardiotoxin) 

    LKCNKLVPLFYKTCPAGKNLCYKMFMVA--KVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
    >2CRT__
    LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
    >2CRS__
    LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
    >1CRF__
    LKCNKLVPLFYKTCPAGKNLCYKMFMVSNLTVPVKRGCIDVCPKNSALVKYVCCNTDRCN
    >1CRE__
    LKCNKLVPLFYKTCPAGKNLCYKMFMVSNLTVPVKRGCIDVCPKNSALVKYVCCNTDRCN
    >2CDX__
    LKCNKLIPIASKTCPAGKNLCYKMFMMSDLTIPVKRGCIDVCPKNSLLVKYVCCNTDRCN
    >1CDT_A
    LKCNKLIPIAYKTCPEGKNLCYKMMLASKKMVPVKRGCINVCPKNSALVKYVCCSTDRCN
    >1CXN__
    LKCNQLIPPFWKTCPKGKNLCYKMTMRAAPMVPVKRGCIDVCPKSSLLIKYMCCNTDKCN
    >1TGX_A
    LKCNQLIPPFWKTCPKGKNLCYKMTMRAAPMVPVKRGCIDVCPKSSLLIKYMCCNTDKCN
    >1CDT_B
    LKCNKLIPIAYKTCPEGKNLCYKMMLASKKMVPVKRGCINVCPKNSALVKYVCCSTDRCN
    >1TGX_C
    LKCNQLIPPFWKTCPKGKNLCYKMTMRAAPMVPVKRGCIDVCPKSSLLIKYMCCNTDKCN
    



(3) Their secondary structure (toxin and cardiotoxin iii) :
  Garnier plot of toxin
     58 aa; DCH = 0, DCS = 0
            .   10    .   20    .   30    .   40    .   50
       LKCNKLVPLFYKTCPAGKNLCYKMFMVAKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
 helix H                 HHHHHHHHHHHHH                           
 sheet     EEEEEEEEE                      EEEEE    EEEEEEEE      
 turns  TTT          TTTT             TTTT     TTTT        TTTTTT
 coil               C                                            

 Residue totals: H: 14   E: 22   T: 21   C:  1
        percent: H: 33.3 E: 52.4 T: 50.0 C:  2.4

  Garnier plot of Cardiotoxin iii (NMR, minimized average structure)
     60 aa; DCH = 0, DCS = 0
            .   10    .   20    .   30    .   40    .   50    .   60
       LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN
 helix H                 HHH     HHHH                              
 sheet     EEEEEEEEE        EEEEE      E E  EEEEE    EEEEEEEE      
 turns  TTT          TTTT            T  T TT     TTTT        TTTTTT
 coil               C                 C                            



(4)
    CHARGE DISTRIBUTIONAL ANALYSIS
 1  0+00+00000 0+00000+00 00+0000000 +000++000- 000+00000+ 000000-+00

 A. CHARGE CLUSTERS.

 Positive charge clusters (cmin = 13/30 or 18/45 or 22/60):  none

 Negative charge clusters:  not evaluated (frequency of - < 5%, too low)

 Mixed charge clusters (cmin = 15/30 or 20/45 or 25/60):  none

 B. HIGH SCORING (UN)CHARGED SEGMENTS.


回到上一頁