(1)Cut Sites by Restriction Enzyme
EcoRI 0 Cut
HinfI G'AnT_C - 1 Cut(s)
152
MseI T'TA_A - 1 Cut(s)
168
Sau3AI 'GATC_ - 3 Cut(s)
39 162 215
Restriction Map of Sequence:
AlwI AciI
CviRI Sau3AI MspA1I Pfl1108I
BccI MaeII BsmI DpnI FauI
\ \ \ \ \ \ \ \ \ \ \
1 aaaaccatcaaatacgttatgctggaatgcaacgaactgatcccgctgttctacgaaacc 60
ttttggtagtttatgcaatacgaccttacgttgcttgactagggcgacaagatgctttgg
^ * ^ * ^ * ^ * ^ * ^ *
K T I K Y V M L E C N E L I P L F Y E T
CviJI
MspI
ScrFI
NciI Pfl1108I
BspMI HphI NlaIII
\ \ \ \ \
61 tgcccggctggtgaaaacatctgctacgaaatgttcatggttgctaccccgaaagttccg 120
acgggccgaccacttttgtagacgatgctttacaagtaccaacgatggggctttcaaggc
^ * ^ * ^ * ^ * ^ * ^ *
C P A G E N I C Y E M F M V A T P K V P
MboII TfiI
MspI
MaeII ScrFI MseI
TaqI NciI Sau3AI BbvI TseI
MaeII CviRI SfaNI HinfI DpnI MaeII Fnu4HI
\ \ \ \ \ \ \ \ \ \ \ \ \ \\
121 tgcgaacgtggttgcatcgacgtttgcccggaatcttctctgatcgttaaatacgtttgc 180
acgcttgcaccaacgtagctgcaaacgggccttagaagagactagcaatttatgcaaacg
^ * ^ * ^ * ^ * ^ * ^ *
C E R G C I D V C P E S S L I V K Y V C
Sau3AI AciI
BsrI HhaI DpnI TauI
BsbI Tsp4CI HaeII Fnu4HI
CviRI BsiEI AlwNI TaqI CviJI
\ \ \\ \ \\ \ \ \ \\
181 tgcaacaccgaccgttgccagtaatccagcgcctgatctctcgaaataaaagccgcatt 240
acgttgtggctggcaacggtcattaggtcgcggactagagagctttattttcggcgtaa
^ * ^ * ^ * ^ * ^ * ^ *
C N T D R C Q Z S S A Z S L E I K A A
(2)polypeptide sequence:
KTIKYVMLECNELIPLFYETCPAGENICYEMFMVATPKVPCERGCIDVCPESSLI
VKYVCCNTDRCQZSSAZSLEIKAA
(3)這是一種新的毒，並沒有其他蛋白質與它一樣，只有類似的蛋白
此蛋白的一些性質：
Query= 8192
(79 letters)
Database: nrl3d.prot
6063 sequences; 1,030,628 total letters.
三種類似的蛋白：
Accession: 2CRS
Cardiotoxin III (NMR, 13 structures) - Chinese cobra
Length = 60
Score = 296 (140.6 bits)
Expect = 2.3e-39
P = 2.3e-39
Identities = 48/59 (81%)
Positives = 58/59 (98%)
Query: 8 LECNELIPLFYETCPAGENICYEMFMVATPKVPCERGCIDVCPESSLI VKYVCCNTDRC 66
L+CN+L+PLFY+TCPAG+N+CY+MFMVATPKVP +RGCIDVCP+SSL+V KYVCCNTDRC
Subject: 1 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSS LLVKYVCCNTDRC 59
------------------------------------------------------------------------
Accession: 2CRT
Cardiotoxin III (NMR, minimized average structure) - Chinese cobra
Length = 60
Score = 296 (140.6 bits)
Expect = 2.3e-39
P = 2.3e-39
Identities = 48/59 (81%)
Positives = 58/59 (98%)
Query: 8 LECNELIPLFYETCPAGENICYEMFMVATPKVPCERGCIDVCPESSLI VKYVCCNTDRC 66
L+CN+L+PLFY+TCPAG+N+CY+MFMVATPKVP +RGCIDVCP+SSL+VK YVCCNTDRC
Subject: 1 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSS LLVKYVCCNTDRC 59
------------------------------------------------------------------------
Accession: 1CRE
Cardiotoxin ii (nmr, minimized average structure) - taiwan cobra
Length = 60
Score = 264 (125.4 bits)
Expect = 8.6e-35
P = 8.6e-35
Identities = 42/59 (71%)
Positives = 54/59 (91%)
Query: 8 LECNELIPLFYETCPAGENICYEMFMVATPKVPCERGCIDVCPESSLI VKYVCCNTDRC 66
L+CN+L+PLFY+TCPAG+N+CY+MFMV+ VP +RGCIDVCP++S +VKYV CCNTDRC
Subject: 1 LKCNKLVPLFYKTCPAGKNLCYKMFMVSNLTVPVKRGCIDVCPKNS ALVKYVCCNTDRC 59
(4)
Number of Hbond = 24
Number of Helices = 1
Number of Strands = 4
Number of Turns = 6
Number of Bridges = 3
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