Bioinformatics-hw8 </tltle> </head> <body BGCOLOR="#FFFFFF"><h1><font color=blue>Homework 8</font></h1> (1)這一段DNA sequence所code的Protein sequence如下︰ W <B>Met</B> P C S G G N <B>Met</B> K K S I L F I F L S V L S F S P F A Q D A K P V E S S K E K I T L E S K K C N I A K K S N K S G P E S <B>Met</B> N S S N Y C C E L C C N P A C T G C Y <B>Stop</B> <B>Stop</B> Y K G N <B>Stop</B> T V P F I F V L I L <B>Met</B> <B>Met</B> S V T Y V L L L C <B>Stop</B> I N R <br>ps.這並不是確定的情形，但是為最可能出現的情形，而以下protein sequence的分析，皆是由第一個Met到第一個stop的sequence來分析 <br><br> (2)這並不是一個新的protein，與下列這個protein具有極高的identity<br> <pre> Escherichia Coli. Heat-stable enterotoxin A4 precursor (STA4). Sequence：MKKSILFIFL SVLSFSPFAQ DAKPVESSKE KITLESKKCN IAKKSNKSGP ESMNSSNYCC ELCCNPACTG CY 72 AA; 7909 MW </pre> <pre> (3)positive residue:10 negative residue:6 <pre> <br> (4) <b>Colour Key:</b><br><BR><font color=blue>AGILPV</font><BR><font color=red>FYW</font><BR><font color=green>DENQRHSTK</font><BR><font color=yellow>CM</font><BR><BR><BR><H3>Your Protein:</H3><BR><font color=yellow>M</font><font color=blue>P</font><font color=yellow>C</font><font color=green>S</font><font color=blue>G</font><font color=blue>G</font><font color=green>N</font><font color=yellow>M</font><font color=green>K</font><font color=green>K</font> <font color=green>S</font><font color=blue>I</font><font color=blue>L</font><font color=red>F</font><font color=blue>I</font><font color=red>F</font><font color=blue>L</font><font color=green>S</font><font color=blue>V</font><font color=blue>L</font> <font color=green>S</font><font color=red>F</font><font color=green>S</font><font color=blue>P</font><font color=red>F</font><font color=blue>A</font><font color=green>Q</font><font color=green>D</font><font color=blue>A</font><font color=green>K</font> <font color=blue>P</font><font color=blue>V</font><font color=green>E</font><font color=green>S</font><font color=green>S</font><font color=green>K</font><font color=green>E</font><font color=green>K</font><font color=blue>I</font><font color=green>T</font><BR><font color=blue>L</font><font color=green>E</font><font color=green>S</font><font color=green>K</font><font color=green>K</font><font color=yellow>C</font><font color=green>N</font><font color=blue>I</font><font color=blue>A</font><font color=green>K</font> <font color=green>K</font><font color=green>S</font><font color=green>N</font><font color=green>K</font><font color=green>S</font><font color=blue>G</font><font color=blue>P</font><font color=green>E</font><font color=green>S</font><font color=yellow>M</font> <font color=green>N</font><font color=green>S</font><font color=green>S</font><font color=green>N</font><font color=red>Y</font><font color=yellow>C</font><font color=yellow>C</font><font color=green>E</font><font color=blue>L</font><font color=yellow>C</font> <font color=yellow>C</font><font color=green>N</font><font color=blue>P</font><font color=blue>A</font><font color=yellow>C</font><font color=green>T</font><font color=blue>G</font><font color=yellow>C</font><font color=red>Y</font><BR> (5) hydrophobicity map for this protein <pre> <center><img src="hydro.gif" weight=650 height=360> <br> Eisenberg hydrophobicity scale with window size 7</center></pre> (6)經過ScanProsite的查詢，得到以下兩筆資料：<br> <pre> [1] <a href="8.htm">PDOC00008</a> <a href="9.htm">PS00008</a> MYRISTYL N-myristoylation site<br><br> [2] <a href="10.htm">PDOC00021</a> <a href="11.htm">PS00022</a> EGF_1<br> EGF-like domain signature 1 <br> <pre> (7)Molecular weight: 8555.9<br> Theoretical pI: 8.66 </pre> <br> </body> </html>