1.使用 GenBank 之 Blast 查詢,鍵入 Sho-Hua 所得之 sequence。 共得到 341 筆資料,其下列 5 筆資料具最高的相似度: dbj|D88622|D88622 Bicistronic retroviral vector 2845 0.0 gb|M28248|SYNMMLPLN4 Moloney murine leukemia virus retroviral v... 2845 0.0 gb|M64753|SYNMOV1 Moloney murine leukemia virus retroviral vect... 2845 0.0 gb|M64754|SYNMOV2 Moloney murine leukemia virus retroviral vect... 2845 0.0 gb|M77239|SYNRRV Cloning vector pLXSH from Moloney murine leuke... 2845 0.0 點選前兩項,作進一步查詢。
(1)Bicistronic retroviral vector 6072 bp DNA 相似 sequence -- 175-1653 sequence 相對 functions-- LTR 175..763 /note="5' LTR" RBS 1649..2263 /note="IRES sequence from ECMV"
(2)Moloney murine leukemia virus retroviral vector pLXSN, complete genome. 5874 bp DNA 相似 sequence -- 175-1653 sequence 相對 functions-- LTR 175..763 /note="Mo-MuSV 5' long terminal repeat" misc_recomb 174^175 /note="mouse DNA end/Mo-MuSV DNA start" misc_signal 833..1642 /note="extended packaging signal" misc_recomb 1163^1164 /note="Mo-MuSV DNA end/Mo-MuLV DNA start" mutation 1223..1225 /note="gag ATG start codon to TAG stop codon"
2.連結至 NCBI,click Taxonomy,鍵入 Lycopersicon esculentum。 得--
(1)Lycopersicon esculentum 其實就是 tomato。
(2)現所知 nucleotide sequence 1273個 protein sequence 1168個
(3)與 heat shock protein 相關者有 21 篇, 其中一篇即為Lycopersicon esculentum class II small heat shock protein。 其 cds 如下-- CDS 108..584 /note="heat treatment/chilling tolerance related protein from tomato fruit" /codon_start=1 /product="class II small heat shock protein Le-HSP17.6" /db_xref="PID:g1773291" /translation="MDLRLLGIDNTPLFHTLHHMMEAAGEDSDKSVNAPSRNYVRDAK AMAATPADVKEYPNSYVFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKFI RMERRVGKFMRKFSLPENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKVA"