1.使用 GenBank 之 Blast 查詢,鍵入 Sho-Hua 所得之 sequence。
共得到 341 筆資料,其下列 5 筆資料具最高的相似度:
dbj|D88622|D88622 Bicistronic retroviral vector 2845 0.0
gb|M28248|SYNMMLPLN4 Moloney murine leukemia virus retroviral v... 2845 0.0
gb|M64753|SYNMOV1 Moloney murine leukemia virus retroviral vect... 2845 0.0
gb|M64754|SYNMOV2 Moloney murine leukemia virus retroviral vect... 2845 0.0
gb|M77239|SYNRRV Cloning vector pLXSH from Moloney murine leuke... 2845 0.0
點選前兩項,作進一步查詢。
(1)Bicistronic retroviral vector
6072 bp DNA
相似 sequence -- 175-1653
sequence 相對 functions--
LTR 175..763
/note="5' LTR"
RBS 1649..2263
/note="IRES sequence from ECMV"
(2)Moloney murine leukemia virus retroviral vector pLXSN, complete genome.
5874 bp DNA
相似 sequence -- 175-1653
sequence 相對 functions--
LTR 175..763
/note="Mo-MuSV 5' long terminal repeat"
misc_recomb 174^175
/note="mouse DNA end/Mo-MuSV DNA start"
misc_signal 833..1642
/note="extended packaging signal"
misc_recomb 1163^1164
/note="Mo-MuSV DNA end/Mo-MuLV DNA start"
mutation 1223..1225
/note="gag ATG start codon to TAG stop codon"
2.連結至 NCBI,click Taxonomy,鍵入 Lycopersicon esculentum。
得--
(1)Lycopersicon esculentum 其實就是 tomato。
(2)現所知 nucleotide sequence 1273個
protein sequence 1168個
(3)與 heat shock protein 相關者有 21 篇,
其中一篇即為Lycopersicon esculentum class II small heat shock protein。
其 cds 如下--
CDS 108..584
/note="heat treatment/chilling tolerance related protein
from tomato fruit"
/codon_start=1
/product="class II small heat shock protein Le-HSP17.6"
/db_xref="PID:g1773291"
/translation="MDLRLLGIDNTPLFHTLHHMMEAAGEDSDKSVNAPSRNYVRDAK
AMAATPADVKEYPNSYVFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKFI
RMERRVGKFMRKFSLPENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKVA"