1. Identify the toxin

   (1) Tool : NCBI BLAST

       可找出下列最相近的 protein sequence ----
     
     sp|P01444|CX3_NAJAT  CYTOTOXIN 3 PRECURSOR (CARDIOTOXIN A...   329  
     
       為 cardiotoxin 3 from Taiwan Cobra
       故並非一個新的 toxin . 

   (2) Tool : ScanProsite

       發現此 sequence 的確具有 
       Snake toxins signature
       -------37-57 GCIDVCPKSSLLVRYVCCNTD-------



2. Sequence homology

    Tool : NCBI BLAST

    所得 homology 最高之前 10 個作 multiple sequence alingment ------
       
  Query:     1 LKCNKLVPLFYKTCPAGKNICYKMFMVATPKLPVKRGCIDVCPKSSLLVRYVCCNTDKCN 60       

  
  S1:          1 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN 60
  [NAJAT CYTOTOXIN 3 PRECURSOR Score = 329 (156.6 bits), Expect = 3.7e-39, P = 3.7e-39, Identities = 56/60 (93%), Positives = 60/60 (100%)]

  S2:        22 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN 81
  [NAJAT CYTOTOXIN 3 PRECURSOR(CTX III) Score = 329 (156.6 bits), Expect = 3.7e-39, P = 3.7e-39, Identities = 56/60 (93%), Positives = 60/60 (100%)]

  S3:        22 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN 81
  [NAJSP CARDIOTOXIN PRECURSOR Score = 329 (156.6 bits), Expect = 3.7e-39, P = 3.7e-39, Identities = 56/60 (93%), Positives = 60/60 (100%)]

  S4:        22 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN 81
  [NAJAT CARDIOTOXIN 3A PRECURSOR Score = 326 (155.2 bits), Expect = 9.9e-39, P = 9.9e-39, Identities = 55/60 (91%), Positives = 60/60 (100%)]

  S5:        22 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKNSLLVKYVCCNTDRCN 81
  [NAJAT CARDIOTOXIN 3B PRECURSOR Score = 324 (154.2 bits), Expect = 1.9e-38, P = 1.9e-38, Identities = 55/60 (91%), Positives = 59/60 (98%)]

  S6:         1 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSALVKYVCCNTDRCN 60
  [NAJNA CYTOTOXIN 2 (CYTOTOXIN II) Score = 320 (152.3 bits), Expect = 7.2e-38, P = 7.2e-38, Identities = 53/60 (88%), Positives = 60/60 (100%)]

  S7:        22 LKCNKLVPLFYKTCPAGKNLCYKMYMVATPKVPVKRGCIDVCPKSSLVLKYVCCNTDRCN 81
  [cardiotoxin III precursor - Chinese cobra Score = 317 (150.9 bits), Expect = 1.9e-37, P = 1.9e-37, Identities = 55/60 (91%), Positives = 59/60 (98%)]

  S8:        22 LKCNKLVPLFYKTCPAGKNLCYKMFMVATPKVPVKRGRIDVCPKSSLLVKYVCCNTDRCN 81
  [cardiotoxin VIII Score = 309 (147.1 bits), Expect = 2.7e-36, P = 2.7e-36, Identities = 52/60 (86%), Positives = 58/60 (96%)]

  S9:         1 LKCNKLIPIASKTCPAGKNLCYKMFMVATPKVPVKRGCIDVCPKSSLLVKYVCCNTDRCN 60
  [NAJKA CYTOTOXIN 5 (CYTOTOXIN II) Score = 308 (146.6 bits), Expect = 3.8e-36, P = 3.8e-36, Identities = 51/60 (85%), Positives = 57/60 (95%)]

  S10:        1 LKCNKLIPLAYKTCPAGKNLCYKMFMVAAPKVPVKRGCIDACPKNSLLVKYVCCNTDRCN 60
  [NAJAT CYTOTOXIN 5 (CARDIOTOXIN T) (CTX5) Score = 307 (146.1 bits), Expect = 5.2e-36, P = 5.2e-36, Identities = 52/60 (86%), Positives = 57/60 (95%)]



3. Structure

    Tool : SWISS-MODEL

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