1. FRP REPRESSOR
- Find the structure of trp
repressor complexed with its operator from PDB. List the following
information:(1) ID number, (2) Auther, (3) Resolution, (4) How
many residues in one monomer, (5) How many cofactors in whole
complex. Show the complex
molecule on your
homepage. Show different color for different chain in cartoon
form. Indicate all the cofactors in spacefill form and blue color.
Show the script that makes the final picture.
- ANSWER:
- (1).1TRO
- (2).Z.OTWINOWSKI,
P.G.ZHANG, P.B.SIGLER
- (3).1.9
Angstroms
- (4).108 residues
2. ARC REPRESSOR
- Transtription of the ant gene during lytic
growth of bacteriophage P22 is regulated by the cooperative
binding of two Arc repressor dimmer to a 21-base-pair operator
site. Please find the co-crystal structure of this Arc
tetramer-operator complex from PDB.
- (a)Make an animation
of whole complex.
- (b)Show the DNA sequence of the Arc reperssor.
- (c)Show three sidechains(gln9,asn11,arg13)
from onemonomer and corresponding DNA bases of them on your
homepage.Color the different sidechains with different color.
Measure the distance between possible hydrogen bonds.