Barley Lipid Transfer Protein

1. The summary information of barley lipid transfer protein

1-1. PDB ID：1LIP
1-2. Experimental method：NMR
1-3. Organism：Hordeum Vulgare
1-4. Organ where this protein come from：Seed
1-5. Location of its four helixs：

HELIX 1：  GLY 4   GLY 19 1 
HELIX 2 ： GLU 26 GLN 37 1
HELIX 3 ： SER 41  ARG 56 1 
HELIX 4 ： LEU 63  CYS 73

1-6. Positions of its four disulfide bonds：

SSBOND 1： CYS 3 CYS 50
SSBOND 2：CYS 13 CYS 27
SSBOND 3：CYS 28 CYS 73
SSBOND 4：CYS 48 CYS 87

1-7.Give the chemical formula of the ligand ：CH3(CH2)14COOH

2.Two different views of this protein by Rasmol：

The ligand is colored by red.

3. Four disulfide bonds of barley lipid transfer protein：

4.The bond length of each disulfide bond and the average bond length of disulfide bond：

The bond length of four disulfide bonds is 6.61, 5.11,5.62, and 6.53.
The  average bond length of disulfide bond is 5.99.

5. The residues in protein that are within 3.0 angstrom of carboxylic group (COO-) of ligand.

CYS 13, LEU 14, MET 10, VAL 17, TRY 16, ILE81, ILE 69