ProtParam tool
User-provided sequence:
1 11 21 31 41 51
| | | | | |
1 MEMVNKIACF VLLCMVVVAP HAEALTCGQV TSTLAPCLPY LMNRGPLGGC CGGVKGLLGQ 60
61 AQTTVDRQTA CTCLKSAASS FTGLDLGKAA SLPSTCSVNI PYKISPSTDC SKVQ
References and documentation are available.
Number of amino acids: 114
Molecular weight: 11715.8
Theoretical pI: 8.36
Amino acid composition:
Ala (A) 11 9.6%
Arg (R) 2 1.8%
Asn (N) 3 2.6%
Asp (D) 3 2.6%
Cys (C) 10 8.8%
Gln (Q) 5 4.4%
Glu (E) 2 1.8%
Gly (G) 10 8.8%
His (H) 1 0.9%
Ile (I) 3 2.6%
Leu (L) 13 11.4%
Lys (K) 6 5.3%
Met (M) 4 3.5%
Phe (F) 2 1.8%
Pro (P) 7 6.1%
Ser (S) 10 8.8%
Thr (T) 10 8.8%
Trp (W) 0 0.0%
Tyr (Y) 2 1.8%
Val (V) 10 8.8%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 5
Total number of positively charged residues (Arg + Lys): 8
Atomic composition:
Carbon C 503
Hydrogen H 834
Nitrogen N 136
Oxygen O 155
Sulfur S 14
Formula: C503H834N136O155S14
Total number of atoms: 1642
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 3625 3435 3290 3160 3000
Abs 0.1% (=1 g/l) 0.309 0.293 0.281 0.270 0.256
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 2900 2800 2690 2560 2400
Abs 0.1% (=1 g/l) 0.248 0.239 0.230 0.219 0.205
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.68
This classifies the protein as unstable.
Aliphatic index: 89.82
Grand average of hydropathicity (GRAVY): 0.430