The computation has been carried out on the complete sequence.
Warning: All computation results shown below do not take into account any
annotated post-translational modification.References
and documentation are available.
Number of amino acids: 348
Molecular weight: 39007.7
Theoretical pI: 5.87
Amino acid composition:
Ala (A) 29 8.3%
Arg (R) 7 2.0%
Asn (N) 15 4.3%
Asp (D) 5 1.4%
Cys (C) 10 2.9%
Gln (Q) 12 3.4%
Glu (E) 17 4.9%
Gly (G) 23 6.6%
His (H) 6 1.7%
Ile (I) 22 6.3%
Leu (L) 28 8.0%
Lys (K) 11 3.2%
Met (M) 16 4.6%
Phe (F) 31 8.9%
Pro (P) 20 5.7%
Ser (S) 15 4.3%
Thr (T) 27 7.8%
Trp (W) 5 1.4%
Tyr (Y) 18 5.2%
Val (V) 31 8.9%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 18
Atomic composition:
Carbon C 1816
Hydrogen H 2722
Nitrogen N 424
Oxygen O 480
Sulfur S 26
Formula: C1816H2722N424O480S26
Total number of atoms: 5468
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 53825 53835 53110 52090 50200
Abs 0.1% (=1 g/l) 1.380 1.380 1.362 1.335 1.287
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 53100 53200 52510 51490 49600
Abs 0.1% (=1 g/l) 1.361 1.364 1.346 1.320 1.272
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.57
This classifies the protein as stable.
Aliphatic index: 90.20
Grand average of hydropathicity (GRAVY): 0.474