ProtParam tool

User-provided sequence:

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ATGAAATCTG ACTTCTACTT CCAGAAATCA GAGCCTCATT CCCTTAGTAG TGAAGCATTG    60
   61 ATGAGGAGAG CAGTGTCTTT GGTAACAGAT AGCACCTCTA CCTTTCTCTC TCAGACCACA   120
  121 TATGCGTTGA TTGAAGCTAT TACTGAATAT ACTAAGGCTG TTTATACCTT AACTTCTCTT   180
  181 TACCGACAAT ATACAAGTTT ACTTGGGAAA ATGAATTCAG AGGAGGAAGA TGAAGTGTGG   240
  241 CAGGTGATCA TAGGAGCCAG AGCTGAGATG ACTTCAAAAC ACCAAGAGTA CTTGAAGCTG   300
  301 GAAACCACTT GGATGACTGC AGTTGGTCTT TCAGAGATGG CAGCAGAAGC TGCATATCAA   360
  361 ACTGGCGCAG ATCAGGCCTC TATAACCGCC AGGAATCACA TTCAGCTGGT GAAACTGCAG   420
  421 GTGGAAGAGG TGCACCAGCT CTCCCGGAAA GCAGAAACCA AGCTGGCAGA AGCACAGATA   480
  481 GAAGAGCTCC GTCAGAAAAC ACAGGAGGAA GGGGAGGAGC GGGCTGAGTC GGAGCAGGAG   540
  541 GCCTACCTGC GTGAGGATTG A

Number of amino acids: 561

Molecular weight: 45305.5

Theoretical pI: 5.19

Amino acid composition:

Ala (A) 173	 30.8%
Arg (R)   0	  0.0%
Asn (N)   0	  0.0%
Asp (D)   0	  0.0%
Cys (C) 117	 20.9%
Gln (Q)   0	  0.0%
Glu (E)   0	  0.0%
Gly (G) 147	 26.2%
His (H)   0	  0.0%
Ile (I)   0	  0.0%
Leu (L)   0	  0.0%
Lys (K)   0	  0.0%
Met (M)   0	  0.0%
Phe (F)   0	  0.0%
Pro (P)   0	  0.0%
Ser (S)   0	  0.0%
Thr (T) 124	 22.1%
Trp (W)   0	  0.0%
Tyr (Y)   0	  0.0%
Val (V)   0	  0.0%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 0

Atomic composition:

Carbon      C	      1660
Hydrogen    H	      2761
Nitrogen    N	       561
Oxygen      O	       686
Sulfur      S	       117

Formula: C1660H2761N561O686S117
Total number of atoms: 5785

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient     8410    7366    6960    6960    6960
Abs 0.1% (=1 g/l)   0.186   0.163   0.154   0.154   0.154



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient        0       0       0       0       0
Abs 0.1% (=1 g/l)   0.000   0.000   0.000   0.000   0.000


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.44
This classifies the protein as unstable.



Aliphatic index: 30.84

Grand average of hydropathicity (GRAVY): 0.817